Genetic variation of Hoplolaimus columbus populations in the United States using PCR-RFLP analysis of nuclear rDNA ITS regions1

C. H. Bae; A.L. Szalanski; R.T. Robbins

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Genetic variation of Hoplolaimus columbus populations in the United States using PCR-RFLP analysis of nuclear rDNA ITS regions1

机译：使用核rDNA ITS区域的PCR-RFLP分析美国霍普莱莫斯哥伦布种群的遗传变异1

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Hoplolaimus columbus is an important nematode pest which causes economic loss of crops including corn, cotton, and soybean in the Southeastern United States. DNA sequences of the ITS1-5.8S-ITS2 region of ribosomal DNA from H. columbus were aligned andanalyzed to characterize intraspecific genetic variation between eleven populations collected from Georgia, Louisiana, North Carolina, and South Carolina. In comparative sequence analysis with clones from either one or two individuals obtained from theeleven populations, we found variability existed among clones from an individual and that clonal diversity observed from within individuals was verified by PCR-RFLP. PCR-RFLP analysis with Rsa I and Msp I restriction enzymes yielded several fragments on3.0% agarose gel that corresponded to different haplotypes in all populations and the sum of digested products exceeded the length of undigested PCR products, which revealed that ITS heterogeneity existed in a genome of H. columbus. This indicates that heterogeneity may play a role in the evolution of this parthenogenetic species.

机译：Hoplolaimus哥伦布是一种重要的线虫害虫，在美国东南部造成包括玉米，棉花和大豆在内的农作物的经济损失。对来自哥伦布氏菌的核糖体DNA的ITS1-5.8S-ITS2区域的DNA序列进行比对和分析，以表征从佐治亚州，路易斯安那州，北卡罗来纳州和南卡罗来纳州收集的11个种群之间的种内遗传变异。在对来自十一个种群的一个或两个个体的克隆进行的比较序列分析中，我们发现一个个体的克隆之间存在变异，并且通过PCR-RFLP验证了从个体内部观察到的克隆多样性。使用Rsa I和Msp I限制性酶的PCR-RFLP分析在3.0％琼脂糖凝胶上产生了多个片段，对应于所有种群中的不同单倍型，消化产物的总和超过了未消化的PCR产物的长度，这表明ITS中存在异质性哥伦布的基因组。这表明异质性可能在该孤雌生殖物种的进化中起作用。

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《Journal of Nematology, with Annual of Applied Nematology》 |2009年第3期|共7页
作者
C. H. Bae; A.L. Szalanski; R.T. Robbins;
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正文语种 eng
中图分类动物分类学（系统动物学）;
关键词
ITS region; PCR-RFLP; haplotypes; Hoplolaimus columbus; lance; nematode;

机译：ITS区域;PCR-RFLP;单倍型;Hoplolaimus columbus;长矛;线虫;

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1. Genetic variation of Hoplolaimus columbus populations in the United States using PCR-RFLP analysis of nuclear rDNA ITS regions1 [J] . C. H. Bae, A.L. Szalanski, R.T. Robbins Journal of Nematology, with Annual of Applied Nematology . 2009,第3期

机译：使用核rDNA ITS区域的PCR-RFLP分析美国霍普莱莫斯哥伦布种群的遗传变异1
2. Genetic variation and phylogenetic analysis of genus Abies distributed in Korea based on RAPD and nuclear rDNA ITS2 sequences [J] . Yong-Hwan Jung, Sang-Hyun Han, You-Sung Oh, The Korean Journal of Genetics . 2000,第4期

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Genetic variation of Hoplolaimus columbus populations in the United States using PCR-RFLP analysis of nuclear rDNA ITS regions1

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