• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Biodegradable alginate microparticles developed by electrohydrodynamic spraying techniques for oral delivery of protein
【24h】

Biodegradable alginate microparticles developed by electrohydrodynamic spraying techniques for oral delivery of protein

机译：通过电动流体动力喷雾技术开发的可生物降解的藻酸盐微粒，用于蛋白质的口服递送

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, alginate microparticles were prepared by cross-linking alginate with calcium chloride solution using an electrohydrodynamic spraying technique. The effects of alginate and calcium chloride concentration as well as electrical potential on particle size and shape were investigated. The results showed that 1 mg ml~(-1) alginate medium viscosity (AMV), 2.5 mg ml~(-1) CaCl2, electrical potential 18 kV (F1) and 0.5 mg ml~(-1) alginate low viscosity (ALV), 2.5 mg ml~(-1) CaCl2, electrical potential 20 kV (F2) yielded the spherical shape and small particles of 937±158nm and 1556 ±51 nm, respectively. In bovine serum albumin (BSA) entrapment efficiency study, initial BSA of 5, 10, 20, 40 and 60% w/w to polymer was incorporated into these alginate microparticles. The results revealed that F2 with initial BSA 10% w/w showed the highest entrapment efficiency of 49.70 ±0.01%. The result of BSA content revealed that F2'with the initial BSA of 20% w/w showed the highest amount of BSA content of 3.92 ± 0.02 mg g~(-1) of particles. F1 and F2 with the initial BSA of 5%, 20% and 40% w/w were chosen to evaluate for the release in PBS pH 7.4. It was found that F1 with the initial BSA of 40% w/w showed the slowest release rate and sustained release. The release of F1 in 0.1 N HCl solution (pH 1.2) was slower than that in pH 7.4. This electrohydrodynamic spray technique (EHDA) can be applied to prepare alginate in micro size and can encapsulate BSA. Alginate microparticles can further be optimized for oral delivery of several pharmaceutical peptides and proteins.
机译：在这项研究中，通过使用电动流体动力喷涂技术将藻酸盐与氯化钙溶液交联来制备藻酸盐微粒。研究了藻酸盐和氯化钙浓度以及电势对粒径和形状的影响。结果表明，1 mg ml〜（-1）海藻酸盐中等粘度（AMV），2.5 mg ml〜（-1）CaCl2，电势18 kV（F1）和0.5 mg ml〜（-1）海藻酸盐低粘度（ALV） ），2.5 mg ml〜（-1）CaCl2、20 kV（F2）的电势分别产生937±158nm和1556±51 nm的球形和小颗粒。在牛血清白蛋白（BSA）包封效率研究中，将初始BSA为聚合物的5％，10％，20％，40％和60％w / w掺入这些藻酸盐微粒中。结果显示，初始BSA为10％w / w的F2表现出最高的包封效率，为49.70±0.01％。牛血清白蛋白含量的结果表明，初始牛血清白蛋白含量为20％w / w的F2'的最高牛血清白蛋白含量为3.92±0.02 mg g〜（-1）。选择初始BSA为5％，20％和40％w / w的F1和F2评估在pH 7.4的PBS中的释放。发现初始BSA为40％w / w的F1显示出最慢的释放速率和持续释放。 F1在0.1 N HCl溶液（pH 1.2）中的释放比pH 7.4中的释放慢。这种电动流体喷涂技术（EHDA）可以用于制备微尺寸的藻酸盐，并且可以包封BSA。海藻酸盐微粒可以进一步优化以用于几种药物肽和蛋白质的口服递送。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号