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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Eudragit nanoparticles loaded with silybin: a detailed study of preparation, freeze-drying condition and in vitro/in vivo evaluation
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Eudragit nanoparticles loaded with silybin: a detailed study of preparation, freeze-drying condition and in vitro/in vivo evaluation

机译：装有水飞蓟宾的Eudragit纳米颗粒：制备，冻干条件和体内/体外评估的详细研究

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摘要

The objective of this work was use of silybin nanoparticles in treatment of ulcerative colitis (UC). Eudragit RL PO nanoparticles loaded with silybin were produced using solvent-evaporation emulsification technique. Then, they were coated by Eudragit FS30D. Drug release was studied in different physiological environments. Colitis was induced by 4% of acetic acid in rats which received freeze-dried nanoparticles of silybin (75 mg/kg/day), dexamethasone (1 mg/kg/day), blank nanoparticles and normal saline orally for 5 days. Then macroscopic, histopathological evaluation and biochemical analysis, including myeloperoxidase (MPO) activity, tumor necrosis factor-a (TNF-alpha) and interleukin-6 (IL-6) levels in colon tissues were determined using enzyme-linked immunosorbent assay (ELISA) kits. Macroscopic and histopathological scores were improved by the optimised nanoparticles. The optimised nanoparticles had a particle size of 109 +/- 6 nm, zeta potential of 15.4 +/- 2 mV, loading efficiency of 98.3 +/- 12% and release efficiency of 40.8 +/- 5.5% at 24 h. TNF-alpha, IL-6 and MPO activity were reduced significantly by nanoparticles compared to control group (p<0.05).
机译：这项工作的目的是使用水飞蓟宾纳米颗粒治疗溃疡性结肠炎（UC）。使用溶剂蒸发乳化技术制备了负载水飞蓟宾的Eudragit RL PO纳米颗粒。然后，将它们用Eudragit FS30D涂覆。在不同的生理环境中研究了药物释放。在大鼠中，口服4％的水飞蓟宾（75 mg / kg / day），地塞米松（1 mg / kg / day），空白纳米颗粒和生理盐水冻干的纳米颗粒诱导大鼠结肠炎5天。然后使用酶联免疫吸附测定（ELISA）确定大肠组织的宏观，组织病理学评估和生化分析，包括髓过氧化物酶（MPO）活性，肿瘤坏死因子-α（TNF-α）和白介素6（IL-6）水平。套件。优化的纳米颗粒改善了宏观和组织病理学评分。优化的纳米粒子的粒径为109 +/- 6 nm，ζ电势为15.4 +/- 2 mV，负载效率为98.3 +/- 12％，24小时释放效率为40.8 +/- 5.5％。与对照组相比，纳米颗粒可显着降低TNF-α，IL-6和MPO活性（p <0.05）。

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