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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Inactive Vibrio cholerae whole-cell vaccine-loaded biodegradable microparticles: in vitro release and oral vaccination
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Inactive Vibrio cholerae whole-cell vaccine-loaded biodegradable microparticles: in vitro release and oral vaccination

机译：灭活的霍乱弧菌全细胞疫苗可生物降解微粒：体外释放和口服疫苗

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An approach is proposed using Vibrio cholerae (VC)-loaded microparticles as oral vaccine delivery systems for improved vaccine bioavailability and increased therapeutic efficacy.The VC-loaded microparticles were prepared with 50:50 poly(DL-lactide-co-glycolide) (PLG),75:25 poly(DL-lactide-co-glycolide) and poly(lactide acid) (PLA)/PEG blend copolymers by the solvent evaporation method.VC was successfully entrapped in three types of microparticles with loading efficiencies and loading levels as follows: 50:50 PLG systems: 97.8% and 55.4 ±6.9 mug/mg; 75:25 PLG systems: 89.2% and 46.5 ±4.4 mug/mg; PLA/PEG-blended systems: 82.6% and 53.7 ± 5.8 mug/mg.The different distributions of VC in the core region and on the surface were as follows: 50:50 PLG systems 25.7 ±1.9 and 6.2 ± 0.9 mug/mg; 75:25 PLG systems: 25.8 ±2.2 and 3.6 ±0.4 (mug/ mg; PLA/PEG-blended systems: 32.4±2.1 and 5.2 ± 1.0 mug/mg,respectively.In vitro active release of VC was affected mainly by matrix type and VC-loaded location in microparticles.The therapeutic immunogenic potential of VC loaded with 50:50 PLG,75:25 PLG and PLA/PEG-blended microparticles was evaluated in adult mice by oral immunization.Significantly higher antibody responses and serum immunoglobin Ig G,IgA and IgM responses were obtained when sera from both VC-loaded 75:25 PLG and PLA/PEG-blended microparticles immunized mice were titrated against VC.The most immuno-genicity in evoking serum IgG,IgA and IgM responses was immunized by VC-loaded PLA/PEG-blended microparticles,and with VC challenge in mice,the survival rate (91.7%).
机译：提出了一种使用霍乱弧菌（VC）微粒作为口服疫苗递送系统的方法，以提高疫苗的生物利用度并提高治疗效果.VC微粒是由50:50聚（DL-丙交酯-乙交酯）（PLG）制备的75:25的聚（DL-丙交酯-共-乙交酯）和聚（丙交酯酸）（PLA）/ PEG共混物通过溶剂蒸发法制备.VC成功地捕获在三种类型的微粒中，其负载效率和负载水平为遵循：50:50 PLG系统：97.8％和55.4±6.9杯/毫克; 75:25 PLG系统：89.2％和46.5±4.4杯/毫克; PLA / PEG共混体系：82.6％和53.7±5.8杯/毫克.VC在核心区域和表面的不同分布如下：50:50 PLG系统25.7±1.9和6.2±0.9杯/毫克; 75:25 PLG系统：25.8±2.2和3.6±0.4（杯子/毫克; PLA / PEG混合系统：32.4±2.1和5.2±1.0杯子/毫克。体外VC的主动释放主要受基质类型影响通过口服免疫评估了成年小鼠中50:50 PLG，75：25 PLG和PLA / PEG混合微粒对VC的治疗免疫原性。抗体反应和血清免疫球蛋白Ig G明显更高，分别从VC加载的75:25 PLG和PLA / PEG混合的免疫小鼠的血清中滴定针对VC的血清，可获得IgA和IgM反应。通过VC免疫引起血清IgG，IgA和IgM反应的免疫原性最高载有PLA / PEG共混物的微粒，并在小鼠中受到VC攻击，存活率达91.7％。

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