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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Comparative study of microcapsules elaborated with three polycations (PLL,PDL,PLO) for cell immobilization
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Comparative study of microcapsules elaborated with three polycations (PLL,PDL,PLO) for cell immobilization

机译：三种聚阳离子（PLL，PDL，PLO）修饰的微胶囊用于细胞固定的比较研究

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Alginate-poly-L-lysine (PLL)-alginate microcapsules have been widely used in cell microencapsulation technology.However,the mechanical fragility and low tensile resistance against swelling of this membrane chemistry and the difficult handling,immunogenicity and cytotoxicity of PLL have stimulated the study of novel polycations.In this paper,alginate microcapsules coated with three different polycations:poly-L-lysine (PLL),poly-D-lysine (PDL) and poly-L-ornithine (PLO) were fabricated to evaluate if the use of novel membrane chemistries (PDL,PLO) had a positive effect on the morphology,osmotic resistance and mechanical stability of the capsules as well as the viability of the immobilized C_2C_(12) myoblast cells when compared to the classical PLL microcapsules.Results indicate that liquefied capsules presented worse mechanical properties than the polymerized solid capsules in the three type of membrane chemistries.In addition,PLL membrane chemistry exerted the highest resistance against compressions after culture in several mediums,while PDL microcapsules showed the highest resistance to the tensile stress of the osmotic pressure.No important differences were detected when the physiological activity of the enclosed cells was evaluated.In summary,although further in vivo assays are needed,in general none of the new membrane formulations represented a significant improvement over classical PLL microcapsules.
机译：藻酸盐-聚-L-赖氨酸（PLL）-藻酸盐微胶囊已广泛用于细胞微囊化技术中。然而，这种膜化学的机械脆性和低抗溶胀性以及难处理的PLL，免疫原性和细胞毒性刺激了藻酸盐。本文研究了用三种不同的聚阳离子包被的藻酸盐微囊：聚-L-赖氨酸（PLL），聚-D-赖氨酸（PDL）和聚-L-鸟氨酸（PLO），以评估其用途。与经典的PLL微胶囊相比，新型膜化学（PDL，PLO）对胶囊的形态，抗渗透性和机械稳定性以及固定化的C_2C_（12）成肌细胞的活力有积极的影响。结果表明：在这三种类型的膜化学中，液化胶囊的力学性能都比聚合的固体胶囊差。此外，PLL膜化学对胶体的抵抗力最高。在多种培养基中培养后压缩，而PDL微囊对渗透压的拉伸应力表现出最高的抵抗力。评估封闭细胞的生理活性时未发现重要差异。总之，尽管需要进一步的体内试验，通常，没有一种新的膜制剂比经典的PLL微囊有明显改善。

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