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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Comparative study of DNA encapsulation into PLGA microparticles using modified double emulsion methods and spray drying techniques
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Comparative study of DNA encapsulation into PLGA microparticles using modified double emulsion methods and spray drying techniques

机译：使用改良的双乳化方法和喷雾干燥技术将DNA包封到PLGA微粒中的比较研究

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Recently,several research groups have shown the potential of microencapsulated DNA as adjuvant for DNA immunization and in tissue engineering approaches.Among techniques generally used for microencapsulation of hydrophilic drug substances into hydrophobic polymers,modified WOW double emulsion method and spray drying of water-in-oil dispersions take a prominent position.The key parameters for optimized microspheres are particle size,encapsulation efficiency,continuous DNA release and stabilization of DNA against enzymatic and mechanical degradation.This study investigates the possibility to encapsulate DNA avoiding shear forces which readily degrade DNA during this microencapsulation.DNA microparticles were prepared with polyethylenimine (PEI) as a complexation agent for DNA.Polycations are capable of stabilizing DNA against enzymatic,as well as mechanical degradation.Further,complexation was hypothesized to facilitate the encapsulation by reducing the size of the macromolecule.This study additionally evaluated the possibility of encapsulating lyophilized DNA and lyophilized DNA/PEI complexes.For this purpose,the spray drying and double emulsion techniques were compared.The size of the microparticles was characterized by laser diffractometry and the particles were visualized by scanning electron microscopy (SEM).DNA encapsulation efficiencies were investigated photometrically after complete hydrolysis of the particles.Finally,the DNA release characteristics from the particles were studied.Particles with a size of < 10 am which represent the threshold for phagocytic uptake could be prepared with these techniques.The encapsulation efficiency ranged from 100-35% for low theoretical DNA loadings.DNA complexation with PEI 25kDa prior to the encapsulation process reduced the initial burst release of DNA for all techniques used.Spray-dried particles without PEI exhibited high burst releases,whereas double emulsion techniques showed continuous release rates.
机译：最近，几个研究小组已经证明了微囊化DNA作为DNA免疫和组织工程方法的佐剂的潜力。在将亲水性药物微囊化为疏水性聚合物，改良的WOW双乳化方法和喷雾干燥水包膜技术中，通常使用了多种技术。油分散体占据重要地位。优化微球的关键参数是粒径，包封效率，连续的DNA释放以及稳定的DNA抵抗酶和机械降解的能力。这项研究探讨了封装DNA的可能性，以避免在剪切过程中容易使DNA降解的剪切力。用聚乙烯亚胺（PEI）作为DNA的络合剂制备了DNA微粒。聚阳离子能够稳定DNA的抗酶促降解和机械降解作用。此外，还提出了通过降低大分子尺寸来促进包封的复杂化方法。这个斯图dy还评估了封装冻干DNA和冻干DNA / PEI复合物的可能性。为此，比较了喷雾干燥和双乳化技术。通过激光衍射对微粒的尺寸进行表征，并通过扫描电子显微镜对微粒进行可视化处理（ SEM）。在颗粒完全水解后，通过光度法研究了DNA的包封效率。最后，研究了颗粒中DNA的释放特性。使用这些技术可以制备粒径小于10 am的颗粒，该颗粒代表吞噬吸收的阈值。对于较低的理论DNA载量，包封效率为100-35％。在包封过程之前，与PEI 25kDa进行的DNA络合降低了所有使用的技术的DNA的突发释放。没有PEI的喷雾干燥颗粒表现出高的突发释放，而两倍乳液技术显示出连续的释放速率。

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