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首页> 外文期刊>Journal of nephrology. >Protective effect of N-acetylcysteine on renal ischemia/reperfusion injury in the rat.
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Protective effect of N-acetylcysteine on renal ischemia/reperfusion injury in the rat.

机译：N-乙酰半胱氨酸对大鼠肾脏缺血/再灌注损伤的保护作用。

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摘要

BACKGROUND: Oxygen free radicals are important components involved in the pathophysiological tissue alterations observed during ischemia/reperfusion (I/R). METHODS: The protective effect of N-acetylcysteine (NAC) against the damage inflicted by reactive oxygen species during renal I/R was investigated in Wistar Albino rats using biochemical parameters. Animals were unilaterally nephrectomized, and subjected to 45 min of renal pedicle occlusion followed by lh of reperfusion. N-acetylcysteine (150 mg/kg, i.p.) or vehicle was administered twice, 15 min prior to ischemia and immediately before the reperfusion period. At the end of the reperfusion period, rats were killed by decapitation. For biochemical analysis, the lipid peroxidation product malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and protein oxidation (PO) were tested. Serum creatinine and BUN concentrations were measured for the evaluation of renal function. RESULTS: I/R induced nephrotoxicity, as evidenced byincreases in BUN and creatinine, was reversed by NAC. The decrease in GSH and increases in MDA, MPO and PO induced by I/R indicated that renal injury involves free radical formation. CONCLUSIONS: Since NAC reversed these oxidant responses, and protected rat renal proximal tubules from in vitro simulated reperfusion injury, it seems that NAC protects kidney tissue against oxidative damage.
机译：背景：氧自由基是缺血/再灌注（I / R）过程中观察到的病理生理组织改变的重要组成部分。方法：使用生化参数研究了Wistar Albino大鼠中N-乙酰半胱氨酸（NAC）对肾脏I / R期间活性氧造成的伤害的保护作用。将动物单侧肾切除，并进行45分钟的肾蒂阻塞，然后再灌注1h。在缺血前15分钟和再灌注期之前，两次施用N-乙酰半胱氨酸（150mg / kg，腹膜内）或赋形剂。在再灌注期结束时，将大鼠断头处死。对于生化分析，测试了脂质过氧化产物丙二醛（MDA）和谷胱甘肽（GSH）的水平，髓过氧化物酶（MPO）活性和蛋白质氧化（PO）。测量血清肌酐和BUN浓度以评估肾功能。结果：NAC逆转了I / R引起的肾毒性，BUN和肌酐的增加证明了这一点。 I / R诱导的GSH降低和MDA，MPO和PO升高表明肾脏损伤涉及自由基形成。结论：由于NAC逆转了这些氧化反应，并保护了大鼠肾近端小管免受体外模拟的再灌注损伤，因此NAC似乎可以保护肾脏组织免受氧化损伤。

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