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首页> 外文期刊>Journal of Micromechanics and Microengineering >Thermal isolation of microchip reaction chambers for rapid non-contact DNA amplification
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Thermal isolation of microchip reaction chambers for rapid non-contact DNA amplification

机译：热隔离微芯片反应室，可快速进行非接触式DNA扩增

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摘要

This paper describes further optimization of a non-contact, infrared-mediated system for microchip DNA amplification via the polymerase chain reaction (PCR). The optimization is focused on heat transfer modeling and subsequent fabrication of thermally isolated reaction chambers in glass devices that are uniquely compatible with non-contact thermal control. With a thermal conductivity approximately an order of magnitude higher than many plastics, glass is not the obvious substrate of choice for rapid thermal cycling in microfluidic chambers, yet it is preferable in terms of optical clarity, solvent compatibility and chemical inertness. Based on predictions of a lumped capacity heat transfer analysis, it is shown here that post-bonding, patterned etching of surrounding glass from microfluidic reaction chambers provides enhancements as high as 3.6-and 7.5-fold in cooling and heating rates, respectively, over control devices with the same chamber designs. These devices are then proven functional for rapid DNA amplification via PCR, in which 25 thermal cycles are completed in only 5 min in thermally isolated PCR chambers of 270 nL volume, representing the fastest static PCR in glass devices reported to date. Amplification of the 500-base pair fragment lambda-DNA was confirmed by capillary gel electrophoresis. In addition to rapid temperature control, the fabrication scheme presented, which is compatible with standard photolithography and wet etching techniques, provides a simple alternative for general thermal management in glass microfluidic devices without metallization.
机译：本文介绍了通过聚合酶链反应（PCR）进行微芯片DNA扩增的非接触式红外介导系统的进一步优化。优化的重点在于传热建模以及玻璃设备中与非接触式热控制唯一兼容的热隔离反应室的后续制造。玻璃的导热系数比许多塑料高大约一个数量级，因此玻璃不是在微流体腔室中进行快速热循环的明显选择基材，但从光学透明度，溶剂相容性和化学惰性方面考虑，它是优选的。根据集总传热分析的预测，此处显示了从微流控反应室进行的后结合，图案化蚀刻的周围玻璃的刻蚀分别使冷却和加热速率分别提高了3.6倍和7.5倍。具有相同腔室设计的设备。这些设备随后被证明可用于通过PCR进行快速DNA扩增，其中在270 nL体积的热隔离PCR箱中仅需5分钟即可完成25个热循环，这是迄今为止报道的玻璃设备中最快的静态PCR。通过毛细管凝胶电泳确认了500个碱基对的片段λ-DNA的扩增。除了快速的温度控制外，与标准的光刻和湿法蚀刻技术兼容的所提出的制造方案为玻璃微流体装置中的常规热管理提供了简单的替代方案，而无需金属化。

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