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首页> 外文期刊>Journal of Microscopy >Light- and electronmicroscopic study of infiltration of resin into initial caries lesions--a new methodological approach.
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Light- and electronmicroscopic study of infiltration of resin into initial caries lesions--a new methodological approach.

机译：用光和电镜研究树脂渗入初始龋损的方法-一种新的方法学方法。

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It was the purpose of this pilot study to investigate resin infiltration into various types of initial subsurface caries lesions using a combined microscopic technique with polarized light microscopy and fluorescence microscopy and subsequent scanning microscopy with EDX-element analysis. Six extracted premolars with initial caries lesions were used. Five were infiltrated with resin after imbibition of the subsurface carious pore volume of enamel with sodium fluorescein solution. After light curing the unbound dye was removed by washing out in water. Serial sections were cut through the lesions and investigated with polarized light microscopy, fluorescence microscopy and simultaneously with both microscopic techniques. The same sections were then studied with scanning electron microscopy and EDX-element analysis to prove the infiltration of the resin into the lesions. The results showed, that the combination technique adds further morphologic information to infiltration behaviour of the resin. The individual volume of early acute lesions versus chronic lesions involving dentin, and the fluorescein bound by resin was well documented in serial sections. The EDX calcium and phosphorus signals correlate negatively with the lesion extension, and the carbon signal correlates positively, thus labelling the resin infiltration. It could be demonstrated that resin infiltration is dependent from the pore volume of the lesion. It can be concluded that the combined polarized light microscopy with fluorescence microscopy is an advantageous tool for studying infiltration of resin into hard tissues.
机译：这项初步研究的目的是使用结合了偏光显微镜和荧光显微镜的显微技术以及随后具有EDX元素分析的扫描显微镜，研究树脂浸润入各种类型的初始地下龋损的情况。使用六种提取的具有初期龋齿损害的前磨牙。用荧光素钠溶液吸收牙釉质的地下龋齿孔体积后，用树脂浸润五种。光固化后，通过用水洗去未结合的染料。切下连续切片穿过病变，并用偏振光显微镜，荧光显微镜以及同时用两种显微镜技术进行研究。然后使用扫描电子显微镜和EDX元素分析研究相同的切片，以证明树脂浸润到病变中。结果表明，结合技术为树脂的渗透行为增加了更多的形态学信息。早期急性病变与慢性病变涉及牙本质的个体体积以及树脂结合的荧光素在连续切片中得到了很好的记录。 EDX钙和磷信号与病变范围呈负相关，而碳信号则呈正相关，从而标记了树脂浸润。可以证明树脂浸润取决于病变的孔体积。可以得出结论，偏振光显微镜与荧光显微镜相结合是研究树脂渗透到硬组织中的有利工具。

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