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首页> 外文期刊>Journal of Neurophysiology >Do neurons from rat neostriatum express both a TTX-sensitive and a TTX-insensitive slow Na+ current?
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Do neurons from rat neostriatum express both a TTX-sensitive and a TTX-insensitive slow Na+ current?

机译：大鼠纹状体的神经元是否同时表达TTX敏感和TTX不敏感的慢Na +电流？

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摘要

1. The properties of a tetrodotoxin (TTX)-sensitive, persistent Na+ current and a purported TTX-insensitive slow Na+ current were studied in acutely isolated neurons from rat neostriatum with the use of the whole cell configuration of the patch-clamp technique. 2. A TTX-sensitive, persistent Na+ current (INaP) was activated positive to -60 mV and reached a peak amplitude of -40 to -120 pA at about -40 mV. As indicated by slow depolarizing voltage ramps, activation of INaP did not require preceding activation of the fast, rapidly inactivating Na+ current. 3. The current-voltage (I-V) relationship of INaP displayed an unexpected inflection after passing through its peak value near -40 mV. Between -40 and -10 mV, INaP declined more rapidly with depolarization than it did at more depolarized potentials. The corresponding conductance (GNaP) peaked at -40 mV and declined to a smaller limiting value at potentials positive to about -10 mV. 4. This behavior is not consistent with the notion that INaP arises solely from a bell-shaped window conductance that results from the overlapping steady-state activation and inactivation curves of the fast Na+ current in a narrow voltage range, nor with the notion that INaP is generated by a single uniform conductance independent of the fast Na+ current. 5. In addition to INaP, a second slow inward current (IS) was evoked when small monovalent cations were omitted from the internal solution. INaP and IS were present both in cells resembling medium spiny neurons and in cells resembling aspiny interneurons. 6. IS was insensitive to TTX (1.2 microM) and the Ca2+ channel blocker, cadmium.(ABSTRACT TRUNCATED AT 250 WORDS)
机译：1.使用膜片钳技术的全细胞配置，在大鼠新纹状体的急性分离神经元中研究了河豚毒素（TTX）敏感的持久性Na +电流和声称的TTX不敏感的缓慢Na +电流的性质。 2. TTX敏感的持续Na +电流（INaP）激活至-60 mV正，并在约-40 mV时达到-40至-120 pA的峰值幅度。如缓慢的去极化电压斜坡所示，INaP的激活不需要先快速激活快速灭活的Na +电流。 3. INaP的电流-电压（I-V）关系在通过其-40 mV附近的峰值后显示出意外的变化。在-40至-10 mV之间，与去极化电势相比，去极化的INaP下降速度更快。相应的电导率（GNaP）在-40 mV时达到峰值，而在约-10 mV的正电位时下降到较小的极限值。 4.这种行为与INaP仅由钟形窗口电导产生的观念不符，INAP只是由于在窄电压范围内快速Na +电流的重叠稳态激活和失活曲线重叠而产生的，也不符合INaP的观念由单一的均匀电导产生，与快速Na +电流无关。 5.除了INaP之外，当内部溶液中省略了小的单价阳离子时，还会引发第二次缓慢的内向电流（IS）。 INaP和IS既存在于类似中棘神经元的细胞中，也存在于像棘间神经元的细胞中。 6. IS对TTX（1.2 microM）和Ca2 +通道阻滞剂镉不敏感。（摘要截短为250字）

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