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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >A universal thermodynamic approach to analyze biomolecular binding experiments.
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A universal thermodynamic approach to analyze biomolecular binding experiments.

机译：分析生物分子结合实验的通用热力学方法。

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摘要

Binding processes of any kind can be characterized as an association of a given ligand with some binding factor. This includes macromolecules as well as supramolecular aggregates such as micelles or membranes. The underlying molecular binding mechanism may be more or less complicated due to various intermediate steps (involving for instance conformational changes, aggregation, cooperativity, etc.). A sensible discussion of possible binding models naturally calls for a model-independent access to basic thermodynamic properties. The present contribution will demonstrate how this can quite generally be accomplished by a pertinent processing of properly selected experimental data. The method requires a series of titration measurements comprising the use of variable amounts of both the ligand and the binding factor. It leads to a linear mass conservation plot (i.e. amount of the ligand vs. a matching amount of the binding factor) whose slope and ordinate intercept are equal to the binding ratio (i.e. bound ligand per binding factor) and the free ligand concentration, respectively. This establishes the specific binding isotherm. The approach also reveals latent structurally determined features of the applied physical measuring signal. A number of examples including specific binding, unspecific adsorption and insertion in two-dimensional molecular films will illustrate the methodology.
机译：任何种类的结合过程都可以表征为给定配体与某种结合因子的缔合。这包括大分子以及超分子聚集体，例如胶束或膜。由于各种中间步骤（例如涉及构象变化，聚集，协同作用等），潜在的分子结合机理可能或多或少复杂。对可能的绑定模型的明智讨论自然要求对模型的基本热力学性质进行独立的访问。本文稿将展示如何通过适当选择适当的实验数据进行适当的处​​理来大致实现这一点。该方法需要一系列滴定测量，包括使用可变数量的配体和结合因子。这导致线性质量守恒图（即配体的量与结合因子的匹配量），其斜率和纵轴截距分别等于结合比（即每个结合因子的结合配体）和游离配体浓度。 。这建立了特定的结合等温线。该方法还揭示了所施加的物理测量信号的潜在结构确定的特征。包括特异性结合，非特异性吸附和在二维分子膜中的插入在内的许多例子将说明该方法。

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