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首页> 外文期刊>Journal of Neurophysiology >Pancreatic polypeptide inhibits calcium channels in rat sympathetic neurons via two signaling pathways.
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Pancreatic polypeptide inhibits calcium channels in rat sympathetic neurons via two signaling pathways.

机译：胰腺多肽通过两个信号通路抑制大鼠交感神经元中的钙通道。

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摘要

1. We studied modulation of N-type Ca2+ channels in adult rat superior cervical ganglion (SCG) neurons by pancreatic polypeptide (PP) using whole cell clamp. In large (> 20 pF) SCG neurons, PP inhibited ICa (35 +/- 2%, mean +/- SE) in a concentration-dependent fashion, with one-half maximal inhibition at 19 nM. 2. One-third of the inhibition was blocked by pertussis toxin, about one-half was blocked by N-ethylmaleimide (NEM) treatments, and about one-half was voltage dependent. The NEM-insensitive component of the PP inhibition was voltage independent and not significantly blocked by intracellular Ca2+ chelators. 3. The NEM-insensitive component was only weakly attenuated by GDP-beta-S, and moderately reversible with guanosine 5'-triphosphate (GTP)-gamma-S, in the whole cell pipette, leaving open the possibility that it is not mediated by a G protein. 4. Hence, PP inhibits ICa via two mechanisms: one G-protein-mediated and the other possibly G-protein independent. The former pathway is sensitive to pertussis toxin (PTX) and NEM, voltage dependent, and shared by several other transmitters in these cells. The latter pathway is PTX-and NEM-insensitive, not voltage dependent, and not affected by the presence of intracellular Ca2+ chelators.
机译：1.我们使用全细胞钳研究了胰腺多肽（PP）对成年大鼠上颈神经节（SCG）神经元中N型Ca2 +通道的调节作用。在大型（> 20 pF）SCG神经元中，PP以浓度依赖的方式抑制ICa（35 +/- 2％，平均+/- SE），最大抑制一半为19 nM。 2.三分之一的抑制作用被百日咳毒素阻断，约一半的抑制作用被N-乙基马来酰亚胺（NEM）处理阻断，约一半的抑制作用与电压有关。 PP抑制的NEM不敏感成分与电压无关，并且不受细胞内Ca2 +螯合剂的明显阻断。 3.在整个细胞移液器中，对NEM不敏感的成分仅被GDP-β-S弱衰减，并且与鸟苷5'-三磷酸（GTP）-γ-S可适度逆转，从而打开了它不被介导的可能性通过G蛋白。 4.因此，PP通过两种机制抑制ICa：一种是G蛋白介导的，另一种可能是G蛋白独立的。前一种途径对百日咳毒素（PTX）和NEM敏感，依赖于电压，并被这些细胞中的其他几种递质共享。后者是PTX和NEM不敏感的，不依赖电压，并且不受细胞内Ca2 +螯合剂的影响。

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