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首页> 外文期刊>Journal of Neurophysiology >Topographical and physiological characterization of interneurons that express engrailed-1 in the embryonic chick spinal cord.
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Topographical and physiological characterization of interneurons that express engrailed-1 in the embryonic chick spinal cord.

机译：在胚胎小鸡脊髓中表达恩化1的中间神经元的地形学和生理学表征。

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A number of homeodomain transcription factors have been implicated in controlling the differentiation of various types of neurons including spinal motoneurons. Some of these proteins are also expressed in spinal interneurons, but their function is unknown. Progress in understanding the role of transcription factors in interneuronal development has been slow because the synaptic connections of interneurons, which in part define their identity, are difficult to establish. Using whole cell recording in the isolated spinal cord of chick embryos, we assessed the synaptic connections of lumbosacral interneurons expressing the Engrailed-1 (En1) transcription factor. Specifically we established whether En1-expressing interneurons made direct connections with motoneurons and whether they constitute a single interneuron class. Cells were labeled with biocytin and subsequently processed for En1 immunoreactivity. Our findings indicate that the connections of En1-expressing cells with motoneurons and with sensory afferents were diverse, suggesting that the population was heterogeneous. In addition, the synaptic connections we tested were similar in interneurons that expressed the En1 protein and in many that did not. The majority of sampled En1 cells did, however, exhibit a direct synaptic connection to motoneurons that is likely to be GABAergic. Because our physiological methods underestimate the number of direct connections with motoneurons, it is possible that the great majority, perhaps all, En1-expressing cells make direct synaptic connections with motoneurons. Our results raise the possibility that En1 could be involved in interneuron-motoneuron connectivity but that its expression is not restricted to a distinct functional subclass of ventral interneuron. These findings constrain hypotheses about the role of En-1 in interneuron development and function.
机译：许多同源域转录因子已经牵涉到控制包括脊髓运动神经元在内的各种类型神经元的分化。这些蛋白质中的一些也在脊髓中神经元中表达，但其功能尚不清楚。由于难以建立部分定义其身份的神经元的突触连接，因此了解转录因子在神经元发育中的作用的进展一直很缓慢。在鸡胚的分离脊髓中使用全细胞记录，我们评估了表达Engrailed-1（En1）转录因子的腰s中间神经元的突触连接。具体来说，我们确定表达En1的中间神经元是否与运动神经元直接建立联系，以及它们是否构成单个中间神经元类。用生物胞素标记细胞，然后处理En1免疫反应性。我们的发现表明，表达En1的细胞与运动神经元和感觉传入的联系是多种多样的，表明该群体是异质的。此外，我们测试的突触连接在表达En1蛋白的中间神经元中相似，而在许多未表达的神经元中。但是，大多数采样的En1细胞确实表现出与运动神经元的直接突触连接，而运动神经元可能是GABA能的。由于我们的生理学方法低估了与运动神经元直接连接的数量，因此，大多数（可能是全部）表达En1的细胞可能与运动神经元直接建立突触连接。我们的结果提高了En1可能参与神经元间运动神经元连通性的可能性，但En1的表达并不局限于腹侧神经元的独特功能亚类。这些发现限制了有关En-1在中间神经元发育和功能中的作用的假设。

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