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首页> 外文期刊>Journal of Neurophysiology >Calcium-independent afterdepolarization regulated by serotonin in anterior thalamus.
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Calcium-independent afterdepolarization regulated by serotonin in anterior thalamus.

机译：血清素调节前丘脑中非钙依赖性的去极化作用。

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Previous studies have identified an afterdepolarization (ADP) in thalamocortical neurons that is mediated by an upregulation of the hyperpolarization-activated current I(h). This ADP has been suggested to play a key role in the generation of spindle oscillations. In the lateral geniculate nucleus, upregulation of I(h) has been shown to be signaled by a rise in intracellular calcium leading to the activation of adenylate cyclase and formation of cAMP. However, it is unclear how generalizable this mechanism is to other thalamic nuclei. We have used whole cell recording to examine the electrophysiological properties of neurons of the anterodorsal thalamic nucleus, a nucleus thought not to undergo spindle oscillations. We now report that cells in this nucleus also display an ADP mediated by I(h). Surprisingly, the ADP and the underlying upregulation of I(h) persisted even after buffering intracellular calcium and blocking calcium influx. These results indicate that, in neurons of the anterodorsal thalamic nucleus, an I(h)-mediated ADP can occur through a mechanism that does not involve a rise in intracellular calcium. We next examined the possibility that this calcium-independent ADP might be modulated by serotonin. Serotonin produced a robust enhancement in the amplitude of the ADP even after strong buffering of intracellular calcium and blockade of calcium channels. These results indicate that neurons of the anterodorsal thalamic nucleus display a calcium-independent, I(h)-mediated ADP and that this ADP is a target for regulation by serotonin. These findings identify a novel mechanism by which serotonin can regulate neuronal excitability.
机译：先前的研究已经确定了由超极化激活电流I（h）上调介导的丘脑皮质神经元的去极化后（ADP）。已建议该ADP在主轴振荡的产生中起关键作用。在外侧膝状细胞核中，I（h）的上调已被表明是由于细胞内钙的升高导致腺苷酸环化酶的活化和cAMP的形成。但是，尚不清楚该机制在其他丘脑核中的适用性。我们已经使用全细胞记录检查了前丘脑前丘脑核神经元的电生理特性，该核被认为不会经历纺锤体振荡。现在我们报告该核中的细胞还显示由I（h）介导的ADP。出人意料的是，即使在缓冲细胞内钙并阻止钙流入之后，ADP和I（h）的潜在上调仍然持续。这些结果表明，在前丘脑丘脑核神经元中，I（h）介导的ADP可以通过不涉及细胞内钙升高的机制发生。接下来，我们研究了5-羟色胺调节这种非钙依赖性ADP的可能性。即使在细胞内钙的强烈缓冲和钙通道的阻滞后，5-羟色胺仍能使ADP的振幅增强。这些结果表明，前丘脑丘脑核神经元显示钙独立的I（h）介导的ADP，并且该ADP是血清素调节的靶标。这些发现确定了5-羟色胺可以调节神经元兴奋性的新机制。

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