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首页> 外文期刊>Journal of Neurophysiology >Somatostatin modulates high-voltage-activated Ca2+ channels in freshly dissociated rat hippocampal neurons.
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Somatostatin modulates high-voltage-activated Ca2+ channels in freshly dissociated rat hippocampal neurons.

机译：生长抑素调节新鲜分离的大鼠海马神经元中的高压激活的Ca2 +通道。

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摘要

1. The effects of somatostatin (SS) on the low-voltage-activated and high-voltage-activated (HVA) Ca2+ channels in pyramidal neurons acutely dissociated from the hippocampal CA1 region of 2- to 3-wk-old rats were investigated in a nystatin perforated-patch recording configuration under voltage-clamp conditions. 2. SS had no effect on the low-voltage-activated Ca2+ channel but did inhibit the HVA Ca2+ channel in a concentration-, time-, and voltage-dependent manner. 3. SS showed the activation phase of Ba2+ current (IBa) passing through HVA Ca2+ channels, and the maximum inhibition was 28% of the total current amplitude measured 10 ms after the current activation. The inhibitory effect was eliminated by applying larger depolarizing prepulses. Pretreatment with pertussis toxin (PTX) completely blocked the effect of SS on HVA IBa, suggesting the contribution of PTX-sensitive Gi/Go proteins to the SS-induced inhibition. 4. The applications of forskolin, 8-Br-cAMP, dibutyryl-guanosine 3'5'-cyclic monophosphate, staurosporine, and 1-(5-isoquinolinylsulphonyl)-2-methylpiperazine did not affect either the control HVA IBa or the SS-induced inhibition of HVA IBa. 5. Pretreatment with protein kinase C (PKC) activators had no significant effect on HVA IBa but did remove the inhibition of HVA IBa by SS. 6. Omega-Conotoxin-GVIA, omega-agatoxin-IVA, nicardipine, and omega-conotoxin-MVIIC blocked HVA IBa by 27, 13, 38, and 9% of the total HVA current, respectively, which suggested the existence of N-, P-, L-, and Q-type HVA Ca2+ channels in the hippocampal CA1 pyramidal neurons.(ABSTRACT TRUNCATED AT 250 WORDS)
机译：1.研究了生长抑素（SS）对2至3周龄大鼠海马CA1区急性分离的锥体神经元的低压激活和高压激活（HVA）Ca2 +通道的影响。电压钳制条件下制霉菌素穿孔膜记录配置。 2. SS对低压激活的Ca2 +通道没有影响，但以浓度，时间和电压相关的方式抑制了HVA Ca2 +通道。 3. SS显示了通过HVA Ca2 +通道的Ba2 +电流（IBa）的激活阶段，最大抑制是电流激活10 ms后测得的总电流幅度的28％。通过施加较大的去极化预脉冲消除了抑制作用。百日咳毒素（PTX）预处理完全阻断了SS对HVA IBa的作用，表明PTX敏感的Gi / Go蛋白对SS诱导的抑制作用有贡献。 4. Forskolin，8-Br-cAMP，二丁酰基-鸟苷3'5'-环一磷酸，星形孢菌素和1-（5-异喹啉基磺酰基）-2-甲基哌嗪的应用均不会影响对照HVA IBa或SS-诱导抑制HVA IBa。 5.用蛋白激酶C（PKC）激活剂预处理对HVA IBa无明显影响，但确实消除了SS对HVA IBa的抑制作用。 6. Omega-Conotoxin-GVIA，omega-agatoxin-IVA，尼卡地平和omega-conotoxin-MVIIC分别阻断了HVA IBa总量的27％，13％，38％和9％，这表明存在N- ，P型，L型和Q型HVA Ca2 +通道位于海马CA1锥体神经元中（摘要截短为250个字）

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