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首页> 外文期刊>Journal of Neurophysiology >Uninjured C-fiber nociceptors develop spontaneous activity and alpha-adrenergic sensitivity following L6 spinal nerve ligation in monkey.
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Uninjured C-fiber nociceptors develop spontaneous activity and alpha-adrenergic sensitivity following L6 spinal nerve ligation in monkey.

机译：猴子的L6脊神经结扎后，未受伤的C纤维伤害感受器会自发活动并产生α-肾上腺素敏感性。

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摘要

We investigated whether uninjured cutaneous C-fiber nociceptors in primates develop abnormal responses after partial denervation of the skin. Partial denervation was induced by tightly ligating spinal nerve L6 that innervates the dorsum of the foot. Using an in vitro skin-nerve preparation, we recorded from uninjured single afferent nerve fibers in the superficial peroneal nerve. Recordings were made from 32 C-fiber nociceptors 2-3 wk after ligation and from 29 C-fiber nociceptors in control animals. Phenylephrine, a selective alpha1-adrenergic agonist, and UK14304 (UK), a selective alpha2-adrenergic agonist, were applied to the receptive field for 5 min in increasing concentrations from 0.1 to 100 microM. Nociceptors from in vitro control experiments were not significantly different from nociceptors recorded by us previously in in vivo experiments. In comparison to in vitro control animals, the afferents found in lesioned animals had 1) a significantly higher incidence of spontaneous activity, 2) a significantly higher incidence of response to phenylephrine, and 3) a higher incidence of response to UK. In lesioned animals, the peak response to phenylephrine was significantly greater than to UK, and the mechanical threshold of phenylephrine-sensitive afferents was significantly lower than for phenylephrine-insensitive afferents. Staining with protein gene product 9.5 revealed an approximately 55% reduction in the number of unmyelinated terminals in the epidermis of the lesioned limb compared with the contralateral limb. Thus uninjured cutaneous C-fiber nociceptors that innervate skin partially denervated by ligation of a spinal nerve acquire two abnormal properties: spontaneous activity and alpha-adrenergic sensitivity. These abnormalities in nociceptor function may contribute to neuropathic pain.
机译：我们调查了灵长类动物中未受伤的皮肤C纤维伤害感受器是否在皮肤部分神经支配后发生异常反应。通过紧密结扎支配足背的脊神经L6诱导部分神经支配。使用体外皮肤神经制剂，我们从腓浅神经中未受伤的单个传入神经纤维中记录下来。在结扎后2-3周从32个C-纤维伤害感受器和对照动物中的29个C-纤维伤害感受器进行记录。苯肾上腺素（一种选择性的α1-肾上腺素能激动剂）和UK14304（英国）（一种选择性的α2肾上腺素能激动剂）以从0.1到100 microM的浓度逐渐增加到受体区域5分钟。来自体外对照实验的伤害感受器与我们先前在体内实验中记录的伤害感受器没有显着差异。与体外对照动物相比，在患病动物中发现的传入神经具有：1）自发活动的发生率显着较高; 2）对去氧肾上腺素的响应发生率显着较高; 3）对UK的响应发生率较高。在患病动物中，对去氧肾上腺素的峰值响应明显大于对UK的响应，对去氧肾上腺素敏感的传入的机械阈值明显低于对去氧肾上腺素不敏感的传入。用蛋白质基因产物9.5染色显示，与对侧肢体相比，病变肢体表皮中未髓鞘末端的数量减少了约55％。因此，未受伤害的皮肤C纤维伤害感受器（通过神经的结扎来神经支配神经）会获得两个异常特性：自发活动和α-肾上腺素敏感性。伤害感受器功能的这些异常可能导致神经性疼痛。

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