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首页> 外文期刊>Journal of Neurophysiology >Membrane properties of chick semicircular canal hair cells in situ during embryonic development.
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Membrane properties of chick semicircular canal hair cells in situ during embryonic development.

机译：雏鸡半圆形管毛细胞在胚胎发育过程中的膜特性。

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The electrophysiological properties of developing vestibular hair cells have been investigated in a chick crista slice preparation, from embryonic day 10 (E10) to E21 (when hatching would occur). Patch-clamp whole-cell experiments showed that different types of ion channels are sequentially expressed during development. An inward Ca(2+) current and a slow outward rectifying K(+) current (I(K(V))) are acquired first, at or before E10, followed by a rapid transient K(+) current (I(K(A))) at E12, and by a small Ca-dependent K(+) current (I(KCa)) at E14. Hair cell maturation then proceeds with the expression of hyperpolarization-activated currents: a slow I(h) appears first, around E16, followed by the fast inward rectifier I(K1) around E19. From the time of its first appearance, I(K(A)) is preferentially expressed in peripheral (zone 1) hair cells, whereas inward rectifying currents are preferentially expressed in intermediate (zone 2) and central (zone 3) hair cells. Each conductance conferred distinctive properties on hair cell voltage response. Starting from E15, some hair cells, preferentially located at the intermediate region, showed the amphora shape typical of type I hair cells. From E17 (a time when the afferent calyx is completed) these cells expressed I(K, L), the signature current of mature type I hair cells. Close to hatching, hair cell complements and regional organization of ion currents appeared similar to those reported for the mature avian crista. By the progressive acquisition of different types of inward and outward rectifying currents, hair cell repolarization after both positive- and negative-current injections is greatly strengthened and speeded up.
机译：从雏鸡第10天（E10）到E21（可能发生孵化），已经在雏鸡crista切片制剂中研究了发育中的前庭毛细胞的电生理特性。膜片钳全细胞实验表明，在发育过程中顺序表达了不同类型的离子通道。首先在E10或之前获取内向Ca（2+）电流和慢速向外整流K（+）电流（I（K（V））），然后获取快速瞬态K（+）电流（I（K （A））），并在E14处施加依赖于Ca的小K（+）电流（I（KCa））。然后，毛细胞的成熟随着超极化激活电流的表达而进行：首先在E16周围出现一个缓慢的I（h），然后在E19周围出现一个快速向内的整流器I（K1）。从首次出现起，I（K（A））优先在外围（1区）毛细胞中表达，而向内整流电流则优先在中间（2区）和中央（3区）毛细胞中表达。每种电导都赋予毛细胞电压响应独特的特性。从E15开始，一些毛发细胞（优先位于中间区域）显示出I型毛发细胞典型的双耳形状。这些细胞从E17（传入花萼完成的时间）开始表达I（K，L），即成熟的I型毛细胞的特征电流。接近孵化的地方，毛细胞的补体和离子流的区域组织似乎与成熟禽cr的报道相似。通过逐步获取不同类型的内向和外向整流电流，正向和负向电流注入后的毛细胞复极化大大增强并加快了速度。

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