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首页> 外文期刊>Journal of Neurophysiology >Spinal NMDA receptors contribute to neuronal processing of acute noxious and nonnoxious colorectal stimulation in the rat.
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Spinal NMDA receptors contribute to neuronal processing of acute noxious and nonnoxious colorectal stimulation in the rat.

机译：脊髓NMDA受体有助于大鼠急性有害和非有害结肠直肠刺激的神经元处理。

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The present study investigated the role of NMDA receptors in the spinal processing of acute noxious and nonnoxious colorectal stimulation using extracellular single-unit recording in the rat. Fifty-three neurons in the L6-S2 dorsal horn of the spinal cord were studied. Neurons were identified using touch and light pinch of the ipsilateral perianal/scrotal area and colorectal distention (CRD). All neurons had excitatory responses to CRD. Thirty neurons were studied using a search stimulus of 80-mmHg CRD. The effects of a systemically administered N-methyl-D-aspartate (NMDA) receptor channel blocker, dizocilpine maleate (MK-801) (0.1, 0.5, 1.0, and 5.0 mg/kg), were tested on the CRD-evoked responses of 13 neurons. The lowest dose had no effect on the neuronal responses to CRD, while greater doses lowered the CRD-evoked responses at all distention pressures tested (20, 40, 60, and 80 mmHg). Similarly, spinal application of MK-801 (20, 50, 100, and 200 nmol) attenuated CRD-evoked activity (n = 9). In addition, a spinally administered competitive NMDA receptor antagonist, 2-amino-5-phosphonovaleric acid (APV) (30, 60, 120, and 240 nmol), dose-dependently attenuated the CRD-evoked response at all distention pressures (n = 5). Systemically administered APV did not affect neuronal responses to CRD (n = 3). Twenty-three neurons were studied in animals that never received distention pressures exceeding 30 mmHg; the search stimulus ranged between 20- and 30-mmHg CRD. These neurons were tested using 20-mmHg CRD. Systemically administered MK-801 facilitated the response to 20-mmHg CRD in three neurons and inhibited the response in five neurons, and the response of five neurons was not affected. Spinally administered MK-801 had no effect on neuronal responses to 20-mmHg CRD in six neurons. However, spinally administered APV dose-dependently decreased the response to 20-mmHg CRD in four neurons. These results are consistent with our previous observations that used Fos expression as the index, suggesting that spinal NMDA receptors contribute to processing of both noxious and nonnoxious CRD.
机译：本研究调查了NMDA受体在大鼠中利用细胞外单单位记录在急性有害和非有害结直肠刺激的脊柱处理中的作用。研究了脊髓L6-S2背角中的五十三个神经元。使用触摸和轻捏同侧肛周/阴囊区域和结直肠扩张（CRD）来识别神经元。所有神经元对CRD都有兴奋性反应。使用80mmHg CRD的搜索刺激研究了30个神经元。测试了全身给药的N-甲基-D-天冬氨酸（NMDA）受体通道阻滞剂马来酸双佐西平（MK-801）（0.1、0.5、1.0和5.0 mg / kg）对CRD引起的CRD应答的影响。 13个神经元。最低剂量对神经元对CRD的反应无影响，而更大剂量可降低在所有测试的扩张压力（20、40、60和80 mmHg）下CRD诱发的反应。同样，脊柱施用MK-801（20、50、100和200 nmol）会减弱CRD诱发的活性（n = 9）。此外，在所有扩张压力下，经脊髓给药的竞争性NMDA受体拮抗剂2-氨基-5-膦酰戊酸（APV）（30、60、120和240 nmol）剂量依赖性地减弱了CRD诱发的反应（n = 5）。全身施用的APV不会影响神经元对CRD的反应（n = 3）。在动物中研究了23个神经元，它们的膨胀压力从未超过30 mmHg。搜索刺激的范围为20至30 mmHg CRD。使用20mmHg CRD测试了这些神经元。全身施用MK-801促进了三个神经元对20mmHg CRD的反应，并抑制了五个神经元的反应，并且五个神经元的反应均未受到影响。脊髓给药的MK-801对六个神经元对20mmHg CRD的神经元反应没有影响。但是，在四个神经元中，脊髓给予APV剂量依赖性地降低了对20 mmHg CRD的反应。这些结果与我们先前使用Fos表达作为指标的观察结果一致，表明脊柱NMDA受体有助于处理有毒和无毒的CRD。

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