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首页> 外文期刊>Journal of Neurophysiology >Spatial distribution and subunit composition of GABA(A) receptors in the inferior olivary nucleus.
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Spatial distribution and subunit composition of GABA(A) receptors in the inferior olivary nucleus.

机译：下橄榄核中GABA（A）受体的空间分布和亚基组成。

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摘要

GABAergic inhibitory feedback from the cerebellum onto the inferior olivary (IO) nucleus plays an important role in olivo-cerebellar function. In this study we characterized the physiology, subunit composition, and spatial distribution of gamma-aminobutyric acid-A (GABA(A)) receptors in the IO nucleus. Using brain stem slices, we identified two types of IO neuron response to local pressure application of GABA, depending on the site of application: a slow desensitizing response at the soma and a fast desensitizing response at the dendrites. The dendritic response had a more negative reversal potential than did the somatic response, which confirmed their spatial origin. Both responses showed voltage dependence characterized by an abrupt decrease in conductance at negative potentials. Interestingly, this change in conductance occurred in the range of potentials wherein subthreshold membrane potential oscillations usually occur in IO neurons. Immunostaining IO sections with antibodies for GABA(A) receptor subunits alpha 1, alpha 2, alpha 3, alpha 5, beta 2/3, and gamma 2 and against the postsynaptic anchoring protein gephyrin complemented the electrophysiological observation by showing a differential distribution of GABA(A) receptor subtypes in IO neurons. A receptor complex containing alpha 2 beta 2/3 gamma 2 subunits is clustered with gephyrin at presumptive synaptic sites, predominantly on distal dendrites. In addition, diffuse alpha 3, beta 2/3, and gamma 2 subunit staining on somata and in the neuropil presumably represents extrasynaptic receptors. Combining electrophysiology with immunocytochemistry, we concluded that alpha 2 beta 2/3 gamma 2 synaptic receptors generated the fast desensitizing (dendritic) response at synaptic sites whereas the slow desensitizing (somatic) response was generated by extrasynaptic alpha 3 beta 2/3 gamma 2 receptors.
机译：小脑对下橄榄核（IO）核的GABA抑制性反馈在小脑小脑功能中起重要作用。在这项研究中，我们表征了IO核中的γ-氨基丁酸-A（GABA（A））受体的生理，亚基组成和空间分布。使用脑干切片，我们根据应用部位确定了对GABA局部施加压力的两种IO神经元反应：在体细胞中缓慢的脱敏反应和在树突处的快速脱敏反应。与体细胞反应相比，树突反应具有更大的负反转潜力，这证实了它们的空间起源。两种响应均显示出电压依赖性，其特征在于在负电位下电导突然降低。有趣的是，这种电导变化发生在电势范围内，其中亚阈值膜电势振荡通常发生在IO神经元中。用针对GABA（A）受体亚基α1，α2，α3，α5，β2/3和γ2的抗体进行免疫染色IO切片，并针对突触后锚蛋白gephyrin进行补充，通过显示GABA的差异分布来补充电生理观察（A）IO神经元中的受体亚型。包含α2β2/3γ2亚基的受体复合物在假定的突触位点（主要在远端树突上）与gephyrin聚集在一起。另外，在躯体上和在神经纤维中的弥漫性α3，β2/ 3和γ2亚基染色可能代表了突触外受体。结合电生理学与免疫细胞化学，我们得出结论，α2β2/3γ2突触受体在突触部位产生快速的脱敏（树突状）反应，而缓慢的脱敏（体细胞）反应由突触外的α3β2/3γ2受体产生。 。

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