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首页> 外文期刊>Journal of Neurophysiology >NR2B-containing NMDA autoreceptors at synapses on entorhinal cortical neurons.
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NR2B-containing NMDA autoreceptors at synapses on entorhinal cortical neurons.

机译：内在皮层神经元的突触中含有NR2B的NMDA自身受体。

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摘要

We have previously shown that presynaptic N-methyl-D-aspartate receptors (NMDARs) can facilitate glutamate release onto principal neurons in the entorhinal cortex (EC). In the present study, we have investigated the subunit composition of these presynaptic NMDARs. We recorded miniature alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor-mediated excitatory postsynaptic currents (mEPSCs), from visually identified neurons in layers II and V of the EC in vitro. In both layers, bath application of the NR2A/B subunit-selective agonist, homoquinolinic acid (HQA), resulted in a marked facilitation of mEPSC frequency. Blockade of presynaptic Ca(2+) entry through either NMDARs or voltage-gated Ca(2+) channels with Co(2+) prevented the effects of HQA, confirming that Ca(2+) entry to the terminal was required for facilitation. When the NR2B-selective antagonist, ifenprodil, was applied prior to HQA, the increase in mEPSC frequency was greatly reduced. In addition, we found that an NMDAR antagonist blocked frequency-dependent facilitation of evoked release and reduced mEPSC frequency in layer V. Thus we have demonstrated that NMDA autoreceptors in layer V of the EC bear the NR2B subunit, and that NMDARs are also present at terminals onto superficial neurons.
机译：我们以前已经表明，突触前N-甲基-D-天冬氨酸受体（NMDARs）可以促进谷氨酸释放到内嗅皮层（EC）中的主要神经元上。在本研究中，我们研究了这些突触前NMDARs的亚基组成。我们记录了微型α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体介导的兴奋性突触后突触电流（mEPSCs），从视觉识别的体外EC层II和V中的神经元。在两层中，NR2A / B亚基选择性激动剂高喹啉酸（HQA）的浴涂均可显着促进mEPSC频率。通过NMDAR或通过电压门控的Ca（2+）通道与Co（2+）阻止突触前Ca（2+）进入阻止了HQA的影响，从而确认需要Ca（2+）进入终端是促进的。在HQA之前使用NR2B选择性拮抗剂ifenprodil时，mEPSC频率的增加大大降低了。此外，我们发现NMDAR拮抗剂阻断了V层中诱发的释放的频率依赖性促进作用，并降低了V层中的mEPSC频率。因此，我们证明了EC第五层中的NMDA自身受体带有NR2B亚基，并且NMDARs也存在于终端到浅层神经元。

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