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首页> 外文期刊>Journal of Neurophysiology >Aging and learning-specific changes in single-neuron activity in CA1 hippocampus during rabbit trace eyeblink conditioning.
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Aging and learning-specific changes in single-neuron activity in CA1 hippocampus during rabbit trace eyeblink conditioning.

机译：兔痕迹眨眼过程中CA1海马中单个神经元活动的衰老和学习特定变化。

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Rabbit trace eyeblink conditioning is a hippocampus-dependent task in which the auditory conditioned stimulus (CS) is separated from the corneal airpuff unconditioned stimulus (US) by a 500-ms empty trace interval. Young rabbits are able to associate the CS and US and acquire trace eyeblink conditioned responses (CRs); however, a subset of aged rabbits show poor learning on this task. Several studies have shown that CA1-hippocampal activity is altered by aging; however, it is unknown how aging affects the interaction of CA1 single neurons within local ensembles during learning. The present study examined the extracellular activity of CA1 pyramidal neurons within local ensembles in aged (29-34 mo) and young (3-6 mo) rabbits during 10 daily sessions (80 trials/session) of trace eyeblink conditioning. A single surgically implanted nonmovable stereotrode was used to record ensembles ranging in size from 2 to 12 separated single neurons. A total of six young and four aged rabbits acquired significant levels of CRs, whereas five aged rabbits showed very few CRs similar to a group of five young pseudoconditioned rabbits. Pyramidal cells (2,159 total) were recorded from these four groups during training. Increases in CA1 pyramidal cell firing to the CS and US were diminished in the aged nonlearners. Local ensembles from all groups contained heterogeneous types of pyramidal cell responses. Some cells showed increases while others showed decreases in firing during the trace eyeblink trial. Hierarchical clustering was used to isolate seven different classes of single-neuron responses that showed unique firing patterns during the trace conditioning trial. The proportion of cells in each group was similar for six of seven response classes. Unlike the excitatory modeling patterns reported in previous studies, three of seven response types (67% of recorded cells) exhibited some type of inhibitory decrease to the CS, US, or both. The single-neuron response classes showed different patterns of learning-related activity across training. Several of the single-neuron types from the aged nonlearners showed unique alterations in response magnitude to the CS and US. Cross-correlation analyses suggest that specific single-neuron types provide more correlated single-neuron activity to the ensemble processing of information. However, aged nonlearners showed a significantly lower level of coincident pyramidal cell firing for all cell types within local ensembles in CA1.
机译：兔痕迹眨眼调节是海马依赖的任务，其中听觉条件刺激（CS）与角膜气喘非条件刺激（US）间隔500毫秒为空。幼兔能够将CS和US联系起来，并获得微量的眨眼条件反应（CR）。但是，一些老龄兔子在此任务上学习能力较差。几项研究表明，CA1-海马活性会随着衰老而改变。但是，尚不清楚衰老如何影响学习过程中局部集合体中CA1单神经元的相互作用。本研究在痕量眨眼调理的每日10天（80次/节）中检查了成年（29-34 mo）和幼年（3-6 mo）兔的局部集合体中CA1锥体神经元的细胞外活性。使用单个手术植入的不可移动立体脚架来记录大小为2至12个分离的单个神经元的合奏。共有六只幼兔和四只成年兔子获得了显着水平的CR，而五只成年兔子显示出的CR却很少，与一组五只年轻的伪条件兔相似。训练期间从这四组中记录了金字塔形细胞（总共2159个）。年龄较大的非学习者的CA1锥体细胞向CS和US发射的增加有所减少。来自所有组的局部合奏包含锥体细胞反应的异质类型。在示踪眨眼试验中，一些细胞显示增加，而其他细胞显示减少。层次聚类用于隔离七种不同类型的单神经元反应，这些反应在痕量条件试验期间显示出独特的激发模式。在七个应答类别中的六个应答类别中，每组中的细胞比例相似。与先前研究报道的兴奋性建模模式不同，七种反应类型中的三种（占记录细胞的67％）表现出对CS，US或两者都有某种抑制作用。单神经反应类在整个训练过程中显示出与学习相关的活动的不同模式。年龄较大的非学习者中的几种单神经元类型对CS和US的反应幅度显示出独特的变化。互相关分析表明，特定的单个神经元类型为信息的整体处理提供了更多相关的单个神经元活动。但是，对于CA1本地集合中的所有细胞类型，年龄较大的非学习者显示出同时发生的锥体细胞激发的水平显着降低。

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