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首页> 外文期刊>Journal of Neuroscience Methods >In vivo chronoamperometric measurements of the clearance of exogenously applied serotonin in the rat dentate gyrus.
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In vivo chronoamperometric measurements of the clearance of exogenously applied serotonin in the rat dentate gyrus.

机译：体内计时电流法测量大鼠齿状回中外源性应用5-羟色胺的清除率。

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摘要

The present study evaluated high-speed chronoamperometry as a method for measuring the clearance of serotonin (5-HT) from extracellular space in vivo. Male Sprague-Dawley rats were anaesthetized and a Nafion-coated, carbon fiber electrode, attached to a multibarrel pipette, was lowered into the subgranular layer of the dentate gyrus, a region which receives dense serotonergic innervation, or the corpus callosum, a fiber tract relatively devoid of the 5-HT transporter (SERT). Serotonin, pressure ejected into these regions, produced replicable electrochemical signals. The amplitude and time course of the signals were significantly prolonged in the corpus callosum compared to the dentate gyrus. Similarly, signals produced by locally applied 5-HT in the dentate gyrus of rats following destruction of hippocampal serotonergic innervation with 5,7-dihydroxytryptamine (5,7-DHT), were significantly enhanced compared to those observed in control animals. The time course of the 5-HT signal was significantly prolonged by local application of the selective 5-HT reuptake inhibitor, fluvoxamine, into the dentate gyrus. By contrast, fluvoxamine did not modify the clearance of 5-HT when locally applied into the dentate gyrus of 5,7-DHT lesioned rats or into the corpus callosum of intact rats. Taken together, these data demonstrate that in intact rats, the SERT contributes to the clearance of exogenously applied 5-HT from the extracellular space. Under the experimental conditions used in this study, high-speed chronoamperometry proved to be a reliable method for directly measuring extracellular 5-HT and appears to be a valuable tool for the study of 5-HT clearance by the SERT in vivo.
机译：本研究评估了高速计时电流法作为一种测量5-羟色胺（5-HT）从体内细胞外间隙清除率的方法。麻醉雄性Sprague-Dawley大鼠，将附着在多桶移液管上的Nafion涂层碳纤维电极降低至齿状回的颗粒下层，该区域接受密集的血清素能神经支配，或称为体，即纤维束。相对缺乏5-HT转运蛋白（SERT）。喷射到这些区域的5-羟色胺产生可复制的电化学信号。与齿状回相比，call体中信号的幅度和时程显着延长。类似地，与在对照动物中观察到的信号相比，在用5,7-二羟基色胺（5,7-DHT）破坏海马血清素能神经支配后，在大鼠齿状回中局部应用5-HT产生的信号明显增强。通过将选择性5-HT再摄取抑制剂氟伏沙明局部应用到齿状回中，可以显着延长5-HT信号的时间过程。相比之下，当氟伏沙明局部应用到5,7-DHT损伤大鼠的齿状回或完整大鼠的call体中时，其不会改变5-HT的清除率。综上所述，这些数据表明，在完整大鼠中，SERT有助于从细胞外间隙清除外源施加的5-HT。在这项研究中使用的实验条件下，高速计时安培法被证明是直接测量细胞外5-HT的可靠方法，并且似乎是通过SERT在体内研究5-HT清除的有价值的工具。

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