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首页> 外文期刊>Journal of Neuroscience Methods >A rapid, easy technique for defining detailed anatomical orientation in pre-deparaffinized tissue sections.
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A rapid, easy technique for defining detailed anatomical orientation in pre-deparaffinized tissue sections.

机译：快速，简便的技术，用于在脱石蜡前的组织切片中定义详细的解剖学方向。

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摘要

During our previous histochemical studies on changes in anterograde and retrograde degeneration following optic nerve transection in rats, it was necessary to make brain sections in which various bilateral components of the visual system were symmetrically included. We discovered a rapid, easy technique that defines a detailed anatomical orientation in pre-deparaffinized tissue sections. The procedure is as follows: several drops of 1% toluidine blue solution are injected with a syringe under the paraffin section on the glass slide after the sections are cut and floated on warm water. After several seconds, the staining solution is removed, and the sections are then ready to be observed under a microscope. The anatomical orientation of the section can easily be defined without deparaffinization. By controlling the condition of sectioning procedures, we could obtain bilateral symmetrical paraffin sections containing all intended components of the visual system. This method is a rapid, simple and reliable way to get tissue sections which contain all of the multiple portions on the same plane.
机译：在我们先前关于大鼠视神经横断后顺行性和逆行性变性变化的组织化学研究中，有必要制作大脑切片，其中对称地包含视觉系统的各个双侧成分。我们发现了一种快速，简便的技术，该技术可在去石蜡前的组织切片中定义详细的解剖学方向。步骤如下：切开切片并将其漂浮在温水中后，用注射器将几滴1％甲苯胺蓝溶液注入玻璃载玻片上的石蜡切片下。几秒钟后，将染色溶液除去，然后准备在显微镜下观察切片。可以在不进行脱石蜡的情况下轻松定义该部分的解剖学方向。通过控制切片程序的条件，我们可以获得包含视觉系统所有预期组成部分的双侧对称石蜡切片。该方法是获得组织切片的快速，简单和可靠的方法，该组织切片包含同一平面上的所有多个部分。

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