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首页> 外文期刊>Journal of Neuroscience Methods >An amperometric glucose-oxidase/poly(o-phenylenediamine) biosensor for monitoring brain extracellular glucose: in vivo characterisation in the striatum of freely-moving rats.
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An amperometric glucose-oxidase/poly(o-phenylenediamine) biosensor for monitoring brain extracellular glucose: in vivo characterisation in the striatum of freely-moving rats.

机译：一种安培型葡萄糖氧化酶/聚（邻苯二胺）生物传感器，用于监测大脑细胞外葡萄糖：自由运动大鼠纹状体的体内特征。

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摘要

Amperometric glucose biosensors based on the immobilization of glucose oxidase (GOx) on Pt electrodes with electropolymerized o-phenylenediamine (PPD) were implanted in the right striatum of freely-moving rats. Carbon paste electrodes for the simultaneous monitoring of ascorbic acid (AA) and/or tissue O2 were implanted in the left striatum. A detailed in vivo characterization of the Pt/PPD/GOx signal was carried out using various pharmacological manipulations. Confirmation that the biosensor responded to changing glucose levels in brain extracellular fluid (ECF) was obtained by intraperitoneal (i.p.) injection of insulin that caused a decrease in the Pt/PPD/GOx current, and local administration of glucose (1 mM) via an adjacent microdialysis probe that resulted in an increase in the biosensor current. An insulin induced increase in tissue O2 in the brain was also observed. Interference studies involved administering AA and subanaesthetic doses of ketamine i.p. Both resulted in increased extracellular AA levels with ketamine also causing an increase in O2. No significant change in the Pt/PPD/GOx current was observed in either case indicating that changes in O2 and AA, the principal endogenous interferents, have minimal effect on the response of these first generation biosensors. Stability tests over a successive 5-day period revealed no significant change in sensitivity. These in vivo results suggest reliable glucose monitoring in brain ECF.
机译：将基于电聚合邻苯二胺（PPD）固定在Pt电极上的葡萄糖氧化酶（GOx）固定的电流型葡萄糖生物传感器植入自由活动大鼠的右纹状体中。用于同时监测抗坏血酸（AA）和/或组织O2的碳糊电极被植入左纹状体中。使用多种药理操作对Pt / PPD / GOx信号进行详细的体内表征。通过腹膜内（ip）注射胰岛素导致Pt / PPD / GOx电流减少，以及通过局部给药葡萄糖（1 mM）来获得对生物传感器对脑细胞外液（ECF）中葡萄糖水平变化的反应的确认。邻近的微透析探针，导致生物传感器电流增加。还观察到胰岛素诱导的脑组织O2增加。干扰研究涉及给予AA和麻醉药剂量的氯胺酮i.p.两者均导致氯胺酮的细胞外AA水平升高，也导致O2升高。在这两种情况下，均未观察到Pt / PPD / GOx电流的显着变化，这表明主要内源性干扰物O2和AA的变化对这些第一代生物传感器的响应影响最小。连续5天的稳定性测试表明，灵敏度没有明显变化。这些体内结果表明对脑ECF中的葡萄糖进行了可靠的监测。

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