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首页> 外文期刊>Journal of Neuroscience Methods >Ultra-violet light-induced changes in membrane properties in secretory cells.
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Ultra-violet light-induced changes in membrane properties in secretory cells.

机译：紫外线诱导分泌细胞膜性质的变化。

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摘要

Illumination with ultra-violet is used widely in physiological experiments for the photolysis of caged compounds. In the peptidergic cells of the pituitary gland, as well as cultured PC12 cells, ultra-violet light was found to produce changes in a number of membrane properties. Light of sufficient intensity to produce rapid photolysis of commonly used caged compounds induced changes in K+ and Ca2+ current, as well as changes in membrane capacitance. All responses to light showed a rapid timecourse, activating in a few ms and decaying within 10-50 ms after illumination ended. Experiments with radical scavengers and with inhibitors of cytochrome p450 and phospholipase A2 failed to block the light responses. These rapid responses to light emphasize that experiments employing ultra-violet light in the photorelease of physiological and pharmacological agents require special care for control of light artifacts.
机译：紫外线照明在笼罩化合物的光解中被广泛用于生理实验。在垂体的肽能细胞以及培养的PC12细胞中，发现紫外线会改变许多膜的性质。具有足够强度的光可以对常用的笼状化合物进行快速光解，从而引起K +和Ca2 +电流的变化以及膜电容的变化。所有对光的反应都显示出快速的时间进程，在照明结束后的几毫秒内激活并在10-50毫秒内衰减。使用自由基清除剂以及细胞色素p450和磷脂酶A2抑制剂的实验未能阻止光反应。这些对光的快速响应强调，在生理剂和药理剂的光释放中采用紫外线的实验需要特别注意控制光伪影。

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