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首页> 外文期刊>Journal of Neuroscience Methods >Two-dimensional photon counting imaging and spatiotemporal characterization of ultraweak photon emission from a rat's brain in vivo.
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Two-dimensional photon counting imaging and spatiotemporal characterization of ultraweak photon emission from a rat's brain in vivo.

机译：二维光子计数成像和体内时空大鼠脑中超弱光子发射的时空特征。

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摘要

The process of metabolic reactions within living cells leads to spontaneous ultraweak light emission. The development of a system for highly sensitive imaging and spatiotemporal analysis of ultraweak photon emission from a rat's brain is reported in this paper. The equipment used in this experiment consists of a two-dimensional photon-counting tube with a photocathode measuring 40 mm in diameter, a highly efficient lens system, and an electronic device to record time series of a photoelectron train with spatial information. The sensitivity and ability to extract spatiotemporal information from sequential data of a single photoelectron train were examined. The minimum detectable radiant flux density of the system was experimentally estimated to be 9.9 x 10(-17) W/cm2 with a 1-s observation time. Spontaneous photon emission was demonstrated from an exposed rat's cortex in vivo without adding any chemical agent or employing external excitation. An image of ultraweak photon emission was compared with one obtained after cardiac arrest. The intensity after cardiac arrest was depressed to approximately 60% of before that. The regional properties of time courses of emission intensity were also demonstrated, indicating the potential usefulness for spatiotemporal characterization of photon emission with mapping of physiological information such as oxidative stress. This technology constitutes a novel method, with the potential to extract pathophysiological information from the central nervous system.
机译：活细胞内的代谢反应过程导致自发的超弱光发射。本文报道了一种用于大鼠大脑超弱光子发射的高灵敏成像和时空分析系统的开发。该实验中使用的设备包括带有直径为40 mm的光电阴极的二维光子计数管，高效的透镜系统以及用于记录具有空间信息的光电子序列的时间序列的电子设备。检查了从单个光电子序列的顺序数据中提取时空信息的敏感性和能力。实验估计该系统的最小可检测辐射通量密度为9.9 x 10（-17）W / cm2，观察时间为1-s。在不添加任何化学试剂或不使用外部激发的情况下，从暴露的大鼠皮层体内证明了自发光子发射。将超弱光子发射的图像与心脏骤停后获得的图像进行比较。心脏骤停后的强度降低到之前的约60％。还证明了发射强度随时间变化的区域特性，表明利用生理信息（例如氧化应激）作图对光子发射时空表征的潜在有用性。该技术构成了一种新颖的方法，具有从中枢神经系统提取病理生理信息的潜力。

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