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首页> 外文期刊>Journal of Neuroscience Methods >Expansion of adult Schwann cells from mouse predegenerated peripheral nerves.
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Expansion of adult Schwann cells from mouse predegenerated peripheral nerves.

机译：从小鼠预先变性的周围神经扩增成年雪旺细胞。

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摘要

We present an effective technique for culture and expansion of Schwann cells (SC) from adult peripheral nerves. Cultures from adult mouse sciatic nerves (one to six nerves per culture) in defined medium showed markedly higher purity and density of SC when the nerve was predegenerated in vivo for 7 days than when it was harvested fresh. SC from degenerated nerves were then cultured in defined media conditioned by primary cultures of adult SC. The best results were obtained with a conditioned medium supplemented with 1% fetal calf serum. In these conditions the purity of SC was about 90% and the density about 190 cell/mm(2) by 7-10 days in vitro. These findings indicate that adult SC can be expanded from small preinjured nerve fragments in a short time period to provide a source of SC for autologous cellular transplants.
机译：我们提出了一种有效的技术，用于从成年周围神经培养和扩增雪旺细胞（SC）。在限定的培养基中，成年小鼠坐骨神经的培养物（每种培养物有1至6条神经）在体内进行7天的自然变性时，其SC的纯度和密度要比新鲜收获时高得多。然后，将变性神经的SC​​培养在成人SC原代培养条件下的特定培养基中。使用补充了1％胎牛血清的条件培养基可获得最佳结果。在这些条件下，到体外培养7-10天，SC的纯度约为90％，密度约为190 cell / mm（2）。这些发现表明，成年SC可以在短时间内从小的受伤前神经碎片中扩增出来，为自体细胞移植提供SC来源。

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