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首页> 外文期刊>Journal of Neuroscience Methods >Use of pseudorabies virus to delineate multisynaptic circuits in brain: opportunities and limitations.
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Use of pseudorabies virus to delineate multisynaptic circuits in brain: opportunities and limitations.

机译：使用伪狂犬病病毒描绘大脑中的多突触回路：机遇与局限。

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摘要

Transsynaptic tracing with live virus is a powerful tool that has been used extensively to analyze central efferents that regulate peripheral targets. More recently, investigators have begun to use this new methodology with central injections to identify circuit anatomy within the brain. Although transsynaptic tracing with peripheral injection of pseudorabies virus has been extensively characterized, several methodological issues related to central application of this tracer have not been addressed. Here, we review the following issues relevant to the use of pseudorabies virus (PRV; Bartha strain) in experiments involving injection of virus into rat brain: (i) factors that determine the zone of viral uptake; (ii) uptake of pseudorabies virus by fibers of passage; (iii) viral invasion of the brain after leakage of virus into the brain ventricles; (iv) considerations for double labeling for PRV with peptides and neurotransmitters; (v) use of PRV with conventional retrograde tracers to anatomically identify relays in a multisynaptic pathway; and (vi) transport of PRV throughout the dendritic tree as a means of identifying inputs to distal dendrites. Collectively, the data demonstrate that PRV provides a powerful means of dissecting the synaptology of CNS circuitry when appropriate controls are incorporated into the experimental design. A set of recipes for various procedures are included at the end of this article.
机译：活病毒的突触追踪是一种功能强大的工具，已广泛用于分析调节外围靶点的中央传出。最近，研究人员已开始将这种新方法与中央注射一起使用，以识别大脑内的回路解剖结构。尽管外周注射假狂犬病病毒的突触追踪已得到广泛表征，但尚未解决与该示踪剂的中心应用有关的一些方法学问题。在这里，我们回顾了与在将病毒注射到大鼠脑中的实验中使用伪狂犬病病毒（PRV; Bartha株）有关的以下问题：（i）确定病毒吸收区域的因素; （ii）通过传播纤维吸收假狂犬病病毒; （iii）病毒泄漏到脑室后病毒侵入脑; （iv）考虑用肽和神经递质双重标记PRV; （v）将PRV与常规逆行示踪剂一起使用，以解剖学方式识别多突触途径中的中转; （vi）PRV在整个树突树中的运输，作为识别远端树突输入的一种手段。总体而言，数据表明，当在实验设计中包含适当的控件时，PRV提供了一种强有力的手段来剖析CNS电路的突触。本文末尾包含了一组用于各种过程的食谱。

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