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首页> 外文期刊>Journal of Neuroscience Methods >A novel technique for micro-dissection of neuronal processes.
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A novel technique for micro-dissection of neuronal processes.

机译:显微解剖神经元过程的新技术。

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摘要

A simple system for cutting dendrites of single neurons without damaging the viability of the cell is described. The system utilizes a rapidly vibrating (100 Hz) micropipette (<1 microm tip diameter), which is dragged under direct visual control across the dendrite of a selected neuron. We used this system in a thin slice preparation to dissect the apical dendrites of rat hippocampal CA1 pyramidal cells. We then used the patch-clamp technique in the whole-cell configuration to record from the isolated somata of these cells and to inject a dye into them. Both a functional and an anatomical disconnection of the dendrites from their somata were verified.
机译:描述了一种在不损害细胞生存力的情况下切割单个神经元树突的简单系统。该系统利用快速振动的(100 Hz)微量移液器(尖端直径<1微米),将其在直接视觉控制下拖动到选定神经元的树突上。我们在薄片制备中使用该系统来解剖大鼠海马CA1锥体细胞的顶端树突。然后,我们在全细胞配置中使用了膜片钳技术,从这些细胞的分离的体细胞中进行记录,并将染料注入其中。树突从其躯体上的功能和解剖上的断开都得到了验证。

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