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首页> 外文期刊>Journal of Neuroscience Methods >Optimization of Golgi methods for impregnation of brain tissue from humans and monkeys.
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Optimization of Golgi methods for impregnation of brain tissue from humans and monkeys.

机译：优化高尔基方法浸渍人和猴子的脑组织的方法。

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摘要

Golgi impregnation is unique in its ability to display the dendritic trees of large numbers of individual neurons. However, its reputation for inconsistency leaves many investigators reluctant to embrace this methodology, particularly for the study of formalin-fixed human brain tissue. After reviewing the literature, testing a variety of technical variations, and discussing the procedure with experienced practitioners, we have concluded that much of the unpredictability can be removed by matching the Golgi technique to the conditions that were used for fixation of the tissue. Briefly fixed tissues worked best with the rapid Golgi technique, which includes osmium during the initial chromation step, and with the Golgi-Cox method, which includes mercuric chloride during chromation. For tissues that have been fixed for several years or even for several decades, superior results are obtained with the Golgi-Kopsch technique, using multiple changes of a chromation solution that contains paraformaldehyde. In the Golgi-Kopsch technique, pH should be used to monitor the reduction of Cr(6+) to Cr(3+), which is a crucial determinant of successful chromation. With any Golgi technique, agitation throughout the impregnation helps to avoid precipitates and to improve the quality of impregnation. When the appropriate method is chosen, Golgi impregnation is a useful technique for the neuropathologist.
机译：高尔基浸渍的独特之处在于它能够显示大量单个神经元的树突树。但是，由于其前后不一致的声誉，许多研究人员不愿采用这种方法，特别是对于福尔马林固定的人脑组织的研究。在回顾了文献，测试了各种技术变化并与经验丰富的从业人员讨论了该程序之后，我们得出结论，可以通过将高尔基技术与用于组织固定的条件相匹配来消除许多不可预测性。使用快速高尔基体技术（在最初的铬化步骤中包括）和高尔基-柯克斯法（其在铬化过程中包括氯化汞）时，简短固定的组织效果最好。对于已经固定了几年甚至几十年的组织，使用高尔基-柯普施技术，通过多次改变含有多聚甲醛的铬酸盐溶液，可以获得优异的结果。在Golgi-Kopsch技术中，应使用pH值来监测Cr（6+）还原为Cr（3+）的情况，这是成功进行铬化的关键决定因素。使用任何高尔基体技术，在整个浸渍过程中进行搅拌有助于避免沉淀，并提高浸渍质量。选择适当的方法后，高尔基浸渍法对于神经病理学家来说是一种有用的技术。

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