Confocal microscopic imaging of fast UV-laser photolysis of caged compounds.

Korkotian E; Oron D; Silberberg Y; Segal M

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Confocal microscopic imaging of fast UV-laser photolysis of caged compounds.

机译：笼状化合物的快速紫外激光光解的共聚焦显微镜成像。

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Using a pulsed UV laser in a confocal scanning microscope, we present a relatively cheap, accurate and efficient method for fast UV laser flash photolysis of caged molecules in two-dimensional cultured neurons. The laser light is introduced through the imaging optics, can be localized by a parallel red laser and can photolyse a sphere of less than 1 microm2, and evoke local fluorescence changes in the imaged neurons. Caged glutamate and caged fluorescein are used to illustrate a disparity between spines and their parent dendrites at a sub-micron resolution.

机译：在共聚焦扫描显微镜中使用脉冲UV激光，我们提出了一种相对便宜，准确和有效的方法，用于二维培养的神经元中笼状分子的快速UV激光快速光解。激光通过成像光学器件引入，可以被平行的红色激光定位，并且可以光解小于1 microm2的球体，并在成像的神经元中引起局部荧光变化。笼状谷氨酸盐和笼状荧光素用于说明亚微米分辨率下的棘突及其亲本树突之间的差异。

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《Journal of Neuroscience Methods》 |2004年第2期|共7页
作者
Korkotian E; Oron D; Silberberg Y; Segal M;
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正文语种 eng
中图分类神经病学与精神病学;
关键词
Lasers; Microscopy; Confocal; Photolysis; 激光; 显微镜检查; 共焦; 光解;

机译：Lasers;Microscopy;Confocal;Photolysis;激光;显微镜检查;共焦;光解;

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1. Confocal microscopic imaging of fast UV-laser photolysis of caged compounds. [J] . Korkotian E, Oron D, Silberberg Y, Journal of Neuroscience Methods . 2004,第1a2期

机译：笼状化合物的快速紫外激光光解的共聚焦显微镜成像。
2. Imaging Single Zno Vertical Nanowire Laser Cavities Using Uv-laser Scanning Confocal Microscopy [J] . Daniel J. Gargas, Maria Eugenia Toimil-Molares, Peidong Yang Journal of the American Chemical Society . 2009,第6期

机译：使用紫外激光扫描共聚焦显微镜对单Zno垂直纳米线激光腔成像
3. Time-resolved electron microscopic analysis of the behavior of myosin heads on actin filaments after photolysis of caged ATP. [J] . T Funatsu, E Kono, S Tsukita Journal of cell biology . 1993,第5期

机译：时间分辨电子显微镜分析笼式ATP光解后肌球蛋白丝上肌球蛋白头的行为。
4. A Fast Approach to Edge Detection for Confocal Microscopic Image [C] . HUANG Xiang-dong, TAN Jiu-bin Instrumentation Science and Technology vol.2 . 2004

机译：共焦显微图像的边缘检测快速方法
5. Ultrafast transient absorption studies of environment influence on the photolysis of B12 complexes and the subsequent recombination and escape of caged radicals [D] . Stickrath, Andrew B. 2008

机译：环境的超快速瞬态吸收研究对B12配合物的光解以及随后的笼状自由基的重组和逸出的影响
6. Photolysis of a Caged Fast-Equilibrating Glutamate Receptor Antagonist MNI-Caged γ-D-Glutamyl-Glycine to Investigate Transmitter Dynamics and Receptor Properties at Glutamatergic Synapses [O] . Francisco Palma-Cerda, George Papageorgiou, Boris Barbour, 2018

机译：笼中的快速平衡的谷氨酸受体拮抗剂MNI笼中的γ-D-谷氨酰-甘氨酸的光解作用以研究谷氨酸能突触中的发射动力学和受体特性。
7. Spatially Complex Diffraction-Limited Photolysis of Caged Calcium and IP3 Combined with High-Speed Confocal Imaging [O] . Shkryl Vyacheslav M., Maxwell Joshua T., Blatter Lothar A. 2011

机译：笼状钙和IP3的空间复杂衍射限制光解结合高速共聚焦成像

Confocal microscopic imaging of fast UV-laser photolysis of caged compounds.

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