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首页> 外文期刊>Journal of Neuroscience Methods >Effective parameters for stimulation of dissociated cultures using multi-electrode arrays.
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Effective parameters for stimulation of dissociated cultures using multi-electrode arrays.

机译:使用多电极阵列刺激解离的培养物的有效参数。

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摘要

Electrical stimulation through multi-electrode arrays is used to evoke activity in dissociated cultures of cortical neurons. We study the efficacies of a variety of pulse shapes under voltage control as well as current control, and determine useful parameter ranges that optimize efficacy while preventing damage through electrochemistry. For any pulse shape, stimulation is found to be mediated by negative currents. We find that positive-then-negative biphasic voltage-controlled pulses are more effective than any of the other pulse shapes tested, when compared at the same peak voltage. These results suggest that voltage-control, with its inherent control over limiting electrochemistry, may be advantageous in a wide variety of stimulation scenarios, possibly extending to in-vivo experiments.
机译:通过多电极阵列的电刺激被用来唤起皮质神经元解离培养物中的活性。我们研究了在电压控制和电流控制下各种脉冲形状的功效,并确定了可优化功效的有用参数范围,同时防止了电化学损害。对于任何脉冲形状,发现刺激都是由负电流介导的。我们发现,当在相同的峰值电压下进行比较时,正-然后-负双相电压控制的脉冲比任何其他测试的脉冲形状更有效。这些结果表明,电压控制及其对限制电化学的固有控制,在多种刺激情况下可能是有利的,可能会扩展到体内实验。

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