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首页> 外文期刊>Journal of Neuroscience Methods >From artificial cerebro-spinal fluid (aCSF) to artificial extracellular fluid (aECF): microdialysis perfusate composition effects on in vivo brain ECF glucose measurements.
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From artificial cerebro-spinal fluid (aCSF) to artificial extracellular fluid (aECF): microdialysis perfusate composition effects on in vivo brain ECF glucose measurements.

机译：从人工脑脊髓液（aCSF）到人工细胞外液（aECF）：微透析灌流液成分对体内脑ECF葡萄糖测量的影响。

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摘要

Microdialysis (mD) is the predominant technique for measurements of brain chemistry, especially when such measurements are made in awake animals. Relatively little attention has been paid, however, to the potential effect on such measurements of variation in the perfusate solution used. Here, we report that small variations in the ionic composition of microdialysis perfusate produced four-fold differences (0.53-2.18mM) in the results obtained when measuring brain extracellular fluid (ECF) glucose. These changes may be linked to concomitant alterations of local neural activity caused by the perfusate composition. In addition to perfusate composition, probe type also proved to have a significant impact on microdialysis measurements. Further, we report the first direct microdialysis measurements of brain ECF ionic composition, showing significant differences from that of the cerebro-spinal fluid (CSF). Modifying the ionic composition of microdialysis perfusate based on these measurements resulted in a measured hippocampal ECF glucose level of 1.26+/-.04mM. Increased understanding of the impact of differences in the perfusate solutions used by different laboratories may provide a basis for reconciliation of apparently disparate microdialysis results within the literature.
机译：微透析（mD）是测量脑化学的主要技术，尤其是在清醒的动物中进行此类测量时。然而，相对较少的注意力集中在对所用灌注液溶液变化的这种测量的潜在影响上。在这里，我们报告微透析灌注液的离子组成中的微小变化在测量脑细胞外液（ECF）葡萄糖时获得的结果产生了四倍的差异（0.53-2.18mM）。这些变化可能与灌注液成分引起的局部神经活动的伴随变化有关。除了灌注液成分外，探针类型还被证明对微透析测量有重要影响。此外，我们报告了脑ECF离子成分的首次直接微透析测量，显示与脑脊髓液（CSF）的显着差异。根据这些测量值修改微透析灌注液的离子组成，导致海马ECF葡萄糖水平为1.26 +/-。04mM。对不同实验室使用的灌注液解决方案差异的影响的加深了解可能为调和文献中明显不同的微透析结果提供基础。

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