...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Neuroscience Methods >A novel primary culture technique for adult retina allows for evaluation of CNS axon regeneration in rodents.
【24h】

A novel primary culture technique for adult retina allows for evaluation of CNS axon regeneration in rodents.

机译：成年视网膜的一种新颖的主要培养技术可以评估啮齿动物中枢神经系统轴突的再生。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Unraveling the causes of regeneration failure in the adult injured CNS has remained a challenge in neurobiology. The notion that CNS neurons lose their regenerative potential during development has been challenged by the identification of several promoters of axon growth. Novel methods are required that allow to study and quantify interactions of molecular determinants, and to envisage future treatment applications. Here we report a novel, highly reproducible method for monitoring axonal regeneration of mature retinal ganglion cells (RGCs) in vitro. In contrast to earlier explantation methods, primary cultures derived from adult rodent retina are kept viable without growth factor supplements. Further, since intraretinal RGC axons remain unmyelinated, regeneration can be followed independently of non-permissive white matter compounds. Applying tracing techniques prior to retinal explantation, cell survival can be correlated to outgrowth activity on the single cell level. Following intervention with pharmacological, growth factor, or gene transfer treatments, retinal explants, and partially RGC neurites, can be processed for protein and gene expression analysis. This novel procedure will prove useful to get insight into complex cell survival and regeneration promoting cascades, and will complement in vivo strategies such as transgenic and knock out mouse models.
机译：揭示成年中枢神经系统损伤的再生失败原因仍然是神经生物学的挑战。识别轴突生长的几个启动子已经挑战了CNS神经元在发育过程中失去再生潜能的观点。需要新颖的方法来研究和量化分子决定簇的相互作用，并设想未来的治疗应用。在这里，我们报告一种新型的，可高度重现的方法，用于监测体外成熟视网膜神经节细胞（RGCs）的轴突再生。与早期的移植方法相反，源自成年啮齿动物视网膜的原代培养物无需添加生长因子即可保持存活。此外，由于视网膜内RGC轴突保持无髓鞘，可以独立于非许可的白质化合物进行再生。在视网膜外植之前应用示踪技术，可以将细胞存活与单细胞水平的生长活动相关。在通过药理学，生长因子或基因转移治疗进行干预后，可以对视网膜外植体和部分RGC神经突进行处理，以进行蛋白质和基因表达分析。这种新颖的方法将证明有助于深入了解复杂的细胞存活和促进级联反应，并将补充体内策略，如转基因和敲除小鼠模型。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号