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首页> 外文期刊>Journal of Neuroscience Methods >Modifications of the Brandel Superfusion 600 system and other revisions in superfusion technique improve the measurement of endogenous dopamine release in vitro.
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Modifications of the Brandel Superfusion 600 system and other revisions in superfusion technique improve the measurement of endogenous dopamine release in vitro.

机译：Brandel Superfusion 600系统的修改和超级融合技术的其他修改改进了体外内源多巴胺释放的测量。

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摘要

The Brandel Superfusion 600 is an automated system designed for the simultaneous measurement of neurotransmitter release from tissue slices from six chambers. In optimizing a technique for the measurement of endogenous dopamine (DA) with this system, a number of factors were identified which affected adversely both the accuracy and the precision of within and between experiments. These factors included bubbles in the perfusion chamber, an apparent loss of tissue viability during preparation of the slices, depletion of DA stores after multiple stimulations and degradation of DA in the perfusate after collection. The method was substantially improved with modifications in the Superfusion 600 system and by the addition of high Mg2+ to the preparation buffer. The addition of tyrosine to the perfusion buffer and the prevention of analyte degradation with antioxidant agents also improved accuracy and precision of measurements.
机译：Brandel Superfusion 600是一个自动化系统，旨在同时测量来自六个腔室的组织切片中神经递质的释放。在使用该系统优化用于测量内源性多巴胺（DA）的技术时，确定了许多因素，这些因素对实验内部和实验之间的准确性和精度均产生不利影响。这些因素包括灌注腔中的气泡，切片制备过程中组织活力的明显丧失，多次刺激后DA存储的耗竭以及收集后灌注液中DA的降解。通过对Superfusion 600系统进行修改并通过向制备缓冲液中添加高Mg2 +，大大改进了该方法。向灌注缓冲液中添加酪氨酸，并使用抗氧化剂防止分析物降解，也提高了测量的准确性和精确度。

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