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首页> 外文期刊>Journal of Neuroscience Methods >A vibrating microtome attachment for cutting brain slice preparations at reproducible compound angles relative to the midline.
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A vibrating microtome attachment for cutting brain slice preparations at reproducible compound angles relative to the midline.

机译：一种振动切片机附件，用于相对于中线以可复制的复合角度切割脑片制剂。

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摘要

Slice preparations isolate functional networks, permitting single unit recording under visual control, and the use of fluorescent indicators. Circuits of interest often lie at a tilt in both the rostrocaudal and ventrodorsal axis, thus exposing circuits of interest at the cut surface of a slice would require a device for tilting a preparation along two orthogonal axes relative to the blade. Such a device, designed to be used in conjunction with a vibrating microtome, permitting the isolation of slice preparations at reproducible angles, is described here. Because the two orthogonal axes of tilt can be independently and continuously adjusted, it is possible to use this device to successively refine tilt parameters from preparation to preparation for optimal exposure of circuits of interest, facilitating the development of new slice preparations. Its use in cutting a thick medullary slab preparation, isolated from the neonate rat, which exposes respiratory networks at the cut surface is described.
机译：切片准备隔离功能网络，允许在视觉控制下记录单个单元，并使用荧光指示剂。感兴趣的电路通常在杆状尾骨和腹背侧的轴线上都倾斜，因此将感兴趣的电路暴露在切片的切割表面处将需要一种装置，用于使制剂沿着两个正交轴相对于刀片倾斜。本文描述了一种设计成与振动切片机结合使用的装置，该装置允许以可再现的角度隔离切片制剂。由于可以独立且连续地调整倾斜的两个正交轴，因此可以使用该设备从准备工作到准备工作相继细化倾斜参数，以实现目标电路的最佳曝光，从而有助于开发新的切片准备工作。描述了其在切割与新生大鼠分离的厚髓质平板制剂中的用途，该制剂在所切割的表面处暴露了呼吸网络。

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