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首页> 外文期刊>Journal of Neuroscience Methods >A near-infrared calibration method suitable for quantification of broadband data in humans.
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A near-infrared calibration method suitable for quantification of broadband data in humans.

机译：一种适用于人类宽带数据量化的近红外校准方法。

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摘要

Broadband near-infrared spectroscopy (bNIRS) is a powerful non-invasive technique for the measurement of hemoglobin. bNIRS systems are relatively simple to construct compared with many near-infrared instruments since they operate on the principle of continuous wave. The advantage of the broadband method is the capacity to model the spectra and to use "the second differential method" to quantify deoxyhemoglobin (HHb). An "anoxia pulse" method can be applied to quantify total haemoglobin (tHb) and tissue oxygen saturation (S(t)O(2)). A disadvantage is that this calibration method is not suitable for application in humans. In this study, we compared the "anoxia pulse" method with "graded hypoxia" method, which can be applied for human studies, to quantify tHb and S(t)O(2). The values obtained with the two methods were respectively (tHb=47.8+/-2.8 and 49.4+/-7.7 microM, mean+/-S.D., n=8) and (S(t)O(2)=72.8+/-3.7% and 73.2+/-5.7%, mean+/-S.D., n=8). There was no significant difference (p<0.05) between the two methods, indicating that the graded hypoxia method could be used for quantification of tHb and S(t)O(2) in human subjects.
机译：宽带近红外光谱法（bNIRS）是一种用于测量血红蛋白的强大非侵入性技术。与许多近红外仪器相比，bNIRS系统的构建相对简单，因为它们的工作原理是连续波。宽带方法的优点是能够对光谱建模并使用“二次微分方法”对脱氧血红蛋白（HHb）进行定量。可以将“缺氧脉冲”方法应用于量化总血红蛋白（tHb）和组织氧饱和度（S（t）O（2））。缺点是该校准方法不适用于人类。在这项研究中，我们将“缺氧脉冲”方法与“分级缺氧”方法进行了比较，该方法可用于人体研究，以定量tHb和S（t）O（2）。两种方法获得的值分别是（tHb = 47.8 +/- 2.8和49.4 +/- 7.7 microM，平均值+/- SD，n = 8）和（S（t）O（2）= 72.8 +/- 3.7 ％和73.2 +/- 5.7％，平均值+/- SD，n ＝ 8）。两种方法之间无显着差异（p <0.05），表明分级缺氧方法可用于定量人类受试者的tHb和S（t）O（2）。

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