...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Neuroscience Methods >Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics
【24h】

Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics

机译：使用线虫和丹尼奥磷酸酶的电压感应域进行遗传编码的荧光电压传感器显示出快速的动力学

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A substantial increase in the speed of the optical response of genetically encoded fluorescent protein voltage sensors (FP voltage sensors) was achieved by using the voltage-sensing phosphatase genes of Nematostella vectensis and Danio rerio. A potential N. vectensis voltage-sensing phosphatase was identified in silico. The voltage-sensing domain (S1-S4) of the N. vectensis homolog was used to create an FP voltage sensor called Nema. By replacing the phosphatase with a cerulean/citrine FRET pair, a new FP voltage sensor was synthesized with fast off kinetics (Tau off5ms). However, the signal was small (ΔF/F=0.4%/200mV). FP voltage sensors using the D. rerio voltage-sensing phosphatase homolog, designated Zahra and Zahra 2, exhibited fast on and off kinetics within 2ms of the time constants observed with the organic voltage-sensitive dye, di4-ANEPPS. Mutagenesis of the S4 region of the Danio FP voltage sensor shifted the voltage dependence to more negative potentials but did not noticeably affect the kinetics of the optical signal.
机译：通过使用vema的线虫和Danio rerio的电压感应磷酸酶基因，大大提高了遗传编码的荧光蛋白电压传感器（FP电压传感器）的光学响应速度。在计算机中鉴定出潜在的维克氏杆菌电压感测磷酸酶。牛肝菌同系物的电压感测域（S1-S4）用于创建称为Nema的FP电压传感器。通过用天青蓝/柠檬色FRET对替代磷酸酶，合成了具有快速关闭动力学（Tau off <5ms）的新型FP电压传感器。但是，信号很小（ΔF/ F = 0.4％/ 200mV）。使用D. rerio电压感应磷酸酶同源物（称为Zahra和Zahra 2）的FP电压传感器在用有机电压敏感染料di4-ANEPPS观察到的时间常数的2ms内显示出快速的开和关动力学。 Danio FP电压传感器S4区域的诱变将电压依赖性转移到更多的负电势，但并未显着影响光信号的动力学。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号