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首页> 外文期刊>Journal of Neuroscience Methods >A stereological method for estimating the total length and size of myelinated fibers in rat cerebral cortex.
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A stereological method for estimating the total length and size of myelinated fibers in rat cerebral cortex.

机译：一种估计大鼠大脑皮层有髓纤维总长度和大小的立体方法。

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摘要

An efficient and unbiased stereological technique to estimate the total length and total volume of the myelinated fibers in rat cortex is described. Unbiased sampling and measuring principles enable to obtain an unbiased estimation of the true value. In the present design, six tissue blocks were obtained from the entire rat brain cortex in a uniform random fashion. Isotropic, uniform random (IUR) sections were ensured by the use of the isector technique. One section with the thickness of 60nm was cut from the center of each epon block using an ultramicrotome. Five fields of vision were photographed using electron microscopy. The length density of the myelinated fibers in the cortex was estimated using an unbiased counting frame. The volume density of the myelinated fibers in the cortex was estimated with point counting. The total length and total volume of the myelinated fibers in the cortex were estimated by multiplying the volume of the cortex, obtained using the Cavalieri principle and the length density and volume density of the fibers in the cortex. The sampling variance introduced by the stereological estimation procedure was a small fraction of the observed total variance indicating an efficient sampling protocol.
机译：描述了一种有效且无偏见的立体技术，用于估计大鼠皮质中有髓纤维的总长度和总体积。无偏采样和测量原理可以使真实值得到无偏估计。在本设计中，以均匀随机的方式从整个大鼠大脑皮层获得了六个组织块。通过使用isector技术，可以确保各向同性，均匀的随机（IUR）截面。使用超薄切片机从每个试块的中心切下一个厚度为60nm的切片。使用电子显微镜拍摄了五个视野。使用无偏计数框架估计皮质中有髓纤维的长度密度。通过点计数估计皮质中有髓纤维的体积密度。皮层中有髓纤维的总长度和总体积是通过将皮层的体积乘以Cavalieri原理获得的，然后乘以皮层中纤维的长度密度和体积密度来估算的。由立体估计程序引入的采样方差是观察到的总方差的一小部分，表明有效的采样方案。

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