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首页> 外文期刊>Journal of Neuroscience Methods >Measurement of intracellular Na+ concentration by a Na+-sensitive fluorescent dye, sodium-binding benzofuran isophthalate, in porcine adrenal chromaffin cells--usage of palytoxin as a Na+ ionophore.
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Measurement of intracellular Na+ concentration by a Na+-sensitive fluorescent dye, sodium-binding benzofuran isophthalate, in porcine adrenal chromaffin cells--usage of palytoxin as a Na+ ionophore.

机译:猪肾上腺嗜铬细胞中通过Na +敏感的荧光染料,钠结合苯并呋喃间苯二甲酸酯测量细胞内Na +的浓度-将草毒素用作Na +离子载体。

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摘要

Palytoxin was found to equilibrate sodium ions (Na+) across the cell membrane much faster than dose gramicidin, which has been frequently used to calibrate the intracellular Na+ concentration ([Na+]in), in cells treated with a Na+-sensitive fluorescent dye, sodium-binding benzofuran isophthalate (SBFI). Palytoxin was capable of equilibrating Na+ in cells treated with SBFI-acetoxymethyl ester (SBFI-AM) and in voltage-clamped cells loaded with SBFI through a patch pipette. Nicotine caused a dose-dependent increase in ([Na+]in) in porcine adrenal chromaffin cells treated with SBFI-AM and caused a simultaneous increase in [Na+]in and inward current in the voltage-clamped cells loaded with SBFI. Palytoxin has an advantage of calibrating ([Na+]in) in a shorter time than dose gramicidin because of its powerful ionophoretic activity.
机译:已发现,Palytoxin在细胞膜上平衡钠离子(Na +)的速度要比剂量短杆菌肽快得多,而在用Na +敏感的荧光染料钠处理过的细胞中,该剂量通常用于校准细胞内Na +浓度([Na +] in)。结合苯二呋喃间苯二甲酸酯(SBFI)。在使用SBFI-乙酰氧基甲基酯(SBFI-AM)处理的细胞中以及通过贴片移液管加载了SBFI的电压钳制细胞中,Palytoxin能够平衡Na +。尼古丁在用SBFI-AM处理的猪肾上腺嗜铬细胞中引起([Na +] in)剂量依赖性增加,并在装有SBFI的电压钳制细胞中同时引起[Na +] in和内向电流增加。由于毒素具有强大的离子电活性,因此它具有比剂量短杆菌肽在更短的时间内校准([Na +] in)的优势。

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