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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >An EPR study of the interfacial properties of phosphatidycholine vesicles with different lipid chain lengths
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An EPR study of the interfacial properties of phosphatidycholine vesicles with different lipid chain lengths

机译：EPR研究不同脂质链长度的磷脂酰胆碱囊泡的界面特性

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摘要

The hydrophobic spin probe 2,2,6,6-tetramethyl-piperidin-1-oxyl-4-yl octadecanoate (TEMPO-stearate) is used to study the interfacial properties of a variety of phosphatidylcholine vesicles. Since the spin probe exhibits a fast motional electron paramagnetic resonance (EPR) spectrum above the phase transition, the EPR spectrum of the spin probe is analyzed by nonlinear least-squares spectral fitting. EPR spectral line fitting provides high precision spectral parameters, which can be used to construct a detailed picture of the dynamics of the probe and its environment. The hyperfine coupling spacing is used to estimate the effective water concentration in the polar shell of vesicles, while the rotational correlation times give the information on the motion of the spin probe. The effective water concentration of the polar shell of dimyristoylphosphatidylglycerol (DMPG) vesicles is greater on average by about 4.0M than the effective water concentration of the polar shell of dimyristoyl-phosphatidylcholine (DMPC) vesicles. The effective water concentration decreases by about 0.5M for an increase of two carbons in the chain, and increases noticeably with hydrocarbon chain unsaturation, which is in good agreement with literature values. The nitroxide moiety rotates preferentially along the N-O bond, that is, parallel to its hydrocarbon chain. (c) 2006 Elsevier B.V. All rights reserved.
机译：疏水自旋探针2,2,6,6-四甲基哌啶-1-氧基-4-基十八烷酸酯（TEMPO-硬脂酸酯）用于研究多种磷脂酰胆碱囊泡的界面性质。由于自旋探针在相变上方显示快速运动电子顺磁共振（EPR）光谱，因此通过非线性最小二乘光谱拟合分析了自旋探针的EPR光谱。 EPR光谱线拟合可提供高精度的光谱参数，可用于构建探针动态及其环境的详细图片。超细耦合间距用于估计囊泡极性壳中的有效水浓度，而旋转相关时间则提供有关自旋探针运动的信息。二肉豆蔻酰基磷脂酰甘油（DMPG）囊泡的极性壳的有效水浓度平均比二肉豆蔻酰基磷脂酰胆碱（DMPC）囊泡的极性壳的有效水浓度平均高约4.0M。随着链中两个碳原子的增加，有效水浓度降低约0.5M，并随着烃链不饱和度的增加而显着增加，这与文献值非常吻合。氮氧化物部分优先沿N-O键旋转，即与其烃链平行。 （c）2006 Elsevier B.V.保留所有权利。

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