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首页> 外文期刊>Journal of Neuroscience Methods >The footfault test as a screening tool in the 6-hydroxydopamine rat model of Parkinson's disease.
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The footfault test as a screening tool in the 6-hydroxydopamine rat model of Parkinson's disease.

机译：脚踩测试作为帕金森氏病6-羟基多巴胺大鼠模型的筛选工具。

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The administration of 6-hydroxydopamine (6-OHDA) into the nigrostriatal pathway is a rat model of Parkinson's disease (PD). The footfault test is a behavioural task in which rodents have their motor functions assessed. Here, we observed that unilaterally 6-OHDA-lesioned animals show a context-induced ipsilateral rotational behaviour when placed on the footfault apparatus for 3min and this may be used as index to detect lesioned animals. Our results showed a sensitivity and specificity of 100% for lesions higher than 94% and 64%, respectively (ROC curve: AUC=0.988). A binary logistic regression model showed an expB=1.116 (95% CI, 1.007-1.236) and C=-9.081+/-4.554 (p=0.046) using the nigral tyrosine hidroxylase immunocontent as standard (each unit represents a 10%-lesion extension). Additionally, the footfault test was more sensitive than apomorphine challenging at 1mg/kg when these tests were carried out days apart and it was less sensitive than methylphenidate at 40mg/kg (sign test, p<0.05). Therefore,the footfault test may be very useful in the PD animal model for screening animals since it is fast and simple and it does not require a drug to induce rotational activity.
机译：将6-羟基多巴胺（6-OHDA）注入黑质纹状体途径是帕金森氏病（PD）的大鼠模型。踩踏测试是一项行为任务，其中啮齿动物需要评估其运动功能。在这里，我们观察到单侧6-OHDA损伤的动物在放于断脚装置上3分钟后表现出上下文相关的同侧旋转行为，这可用作检测病灶动物的指标。我们的结果显示，对于高于94％和64％的病变，敏感性和特异性分别为100％（ROC曲线：AUC = 0.988）。二进制logistic回归模型显示以黑酪氨酸羟化酶免疫含量为标准（每个单位代表10％病变）的expB = 1.116（95％CI，1.007-1.236）和C = -9.081 +/- 4.554（p = 0.046）延期）。此外，当这些试验分开进行数天时，踩踏试验比1mg / kg的阿扑吗啡更具挑战性，而对40mg / kg的哌醋甲酯的敏感性较弱（符号试验，p <0.05）。因此，踩脚试验在PD动物模型中用于筛选动物可能非常有用，因为它既快速又简单，并且不需要药物诱导旋转活动。

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