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首页> 外文期刊>Journal of Neuroscience Methods >Tracheotomy improves experiment success rate in mice during urethane anesthesia and stereotaxic surgery.
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Tracheotomy improves experiment success rate in mice during urethane anesthesia and stereotaxic surgery.

机译：气管切开术可提高氨基甲酸乙酯麻醉和立体定向手术中小鼠的实验成功率。

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摘要

Urethane anesthesia is frequently used for acute experiments on small rodents in physiology and neuroscience. Severe respiratory distress is a common side-effect of urethane anesthesia in many strains of mice. Associated complications interfere with completion of experiments, and as a consequence more animals must be sacrificed. During experiments with stereotaxic brain surgery, we found that intubation by means of tracheotomy is an efficient way to maintain patent airways in these animals. Artificial ventilation of the animals is not required. In this paper we describe a simple, fast and reliable method for intubation of mice in experiments that involve a stereotaxic instrument. The method proved considerably easier to learn and apply than conventional intubation through the oral route. The incidence of breathing problems decreased from 77% in untreated mice to 9% in those that underwent tracheotomy. In addition, the success rate for our acute electrophysiological experiments increased from 24 to 77%.We conclude that tracheotomy reduces the number of sacrificed animals, and saves time and labor.
机译：尿烷麻醉常用于生理和神经科学领域的小型啮齿动物的急性实验。在许多小鼠品系中，严重的呼吸窘迫是氨基甲酸乙酯麻醉的常见副作用。相关的并发症干扰了实验的完成，因此必须处死更多的动物。在立体定向脑外科手术实验中，我们发现通过气管切开术进行插管是维持这些动物气道通畅的有效方法。不需要人工通风。在本文中，我们描述了一种在涉及立体定位仪的实验中对小鼠进行插管的简单，快速和可靠的方法。与通过口腔途径进行的常规插管相比，该方法被证明易于学习和应用。呼吸问题的发生率从未经治疗的小鼠的77％降低到接受气管切开术的小鼠的9％。此外，我们的急性电生理实验的成功率从24％提高到77％。我们得出的结论是，气管切开术减少了处死动物的数量，节省了时间和劳力。

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