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首页> 外文期刊>Journal of Neuroscience Methods >Cocaine-induced Fos expression is detectable in the frontal cortex and striatum of rats under isoflurane but not alpha-chloralose anesthesia: implications for FMRI.
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Cocaine-induced Fos expression is detectable in the frontal cortex and striatum of rats under isoflurane but not alpha-chloralose anesthesia: implications for FMRI.

机译：可卡因诱导的Fos表达在异氟烷下可在大鼠的额叶皮层和纹状体中检测到，但在α-氯代海洛因麻醉下则未检测到：对FMRI的影响。

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摘要

The ability of intravenous cocaine to induce Fos protein expression in anesthetized rats was tested. Two anesthetic regimens commonly used for in vivo FMRI of animals, i.v. alpha-chloralose and gaseous isoflurane, were studied in separate cohorts. The first experiment included three groups that received the following treatments: saline i.v. and no anesthetic; 2 mg/kg cocaine i.v. and no anesthetic; and 2mg/kg cocaine i.v. under 36 mg/kg/h alpha-chloralose anesthesia. The second experiment had a factorial design of four groups that were either nonanesthetized or isoflurane-treated and were either given saline or cocaine (2 mg/kg, i.v.). Anesthetized rats were maintained for 2 h under 2.5-3.5% isoflurane anesthesia, while nonanesthetized rats were kept in an alternative environment for the same time period. Rats were given 2 mg/kg cocaine or saline i.v., 30 min into the test session. Rats were perfused and their brains were processed for Fos immunohistochemistry 90 min after the i.v. treatment. In both experiments, the frontal cortex and striatum of the cocaine-treated nonanesthetized rats expressed Fos in greater amounts than the saline-treated nonanesthetized rats, as expected. The alpha-chloralose treatment prevented cocaine-induced Fos expression across all eight subregions of the striatum and frontal cortex that were examined. In contrast, isoflurane only partially attenuated Fos expression in the orbital and Cg2 subregions of frontal cortex. These results suggest a strong advantage for using isoflurane, as opposed to alpha-chloralose, when studying anesthetized rats for in vivo effects of psychostimulants.
机译：测试了静脉注射可卡因在麻醉大鼠中诱导Fos蛋白表达的能力。通常用于动物体内FMRI的两种麻醉方案在单独的队列中研究了α-氯藻糖和气态异氟烷。第一个实验包括接受以下治疗的三组：生理盐水。而且没有麻醉药; i.v. 2 mg / kg可卡因而且没有麻醉药;和2mg / kg可卡因静脉注射低于36 mg / kg / h的α-氯代醛麻醉。第二个实验的析因设计分为四组，分别进行非麻醉或异氟烷处理，并给予生理盐水或可卡因（2 mg / kg，静脉内）。麻醉的大鼠在2.5-3.5％的异氟烷​​麻醉下维持2小时，而未麻醉的大鼠在相同的时间段内保持在替代环境中。在测试期间的30分钟内，给大鼠静脉注射2 mg / kg可卡因或生理盐水。静脉内注射90分钟后，对大鼠进行灌注并对其大脑进行Fos免疫组织化学处理。治疗。在两个实验中，可卡因处理的非麻醉大鼠的额叶皮层和纹状体表达的Fos均比盐水处理的非麻醉大鼠大，如预期的那样。 α-氯藻糖处理可预防可卡因诱导的在纹状体和额叶皮层的所有八个子区域中的Fos表达。相反，异氟烷仅部分减弱额叶皮质的眼眶和Cg2子区域的Fos表达。这些结果表明，在研究麻醉的大鼠对精神刺激药的体内作用时，与使用α-氯藻酸酯相比，使用异氟烷具有很大的优势。

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