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首页> 外文期刊>Journal of Neuroscience Methods >A method for measuring brain partial pressure of oxygen in unanesthetized unrestrained subjects: the effect of acute and chronic hypoxia on brain tissue PO(2).
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A method for measuring brain partial pressure of oxygen in unanesthetized unrestrained subjects: the effect of acute and chronic hypoxia on brain tissue PO(2).

机译：一种在未麻醉且不受约束的受试者中测量大脑的脑氧分压的方法：急性和慢性低氧对脑组织PO（2）的影响。

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摘要

The level of tissue oxygenation provides information related to the balance between oxygen delivery, oxygen utilization, tissue reactivity and morphology during physiological conditions. Tissue partial pressure of oxygen (PtO(2)) is influenced by the use of anesthesia or restraint. These factors may impact the absolute level of PtO(2). In this study we present a novel fiber optic method to measure brain PtO(2). This method can be used in unanesthetized, unrestrained animals, provides absolute values for PO(2), has a stable calibration, does not consume oxygen and is MRI compatible. Brain PtO(2) was studied during acute hypoxia, as well as before and after 28 days of high altitude acclimatization. A sensor was chronically implanted in the frontal cortex of eight Wistar rats. It is comprised of a fiber optic probe with a tip containing material that fluoresces with an oxygen dependent lifetime. Brain PtO(2) declines by 80% and 76% pre- and post-acclimatization, respectively, when the fraction of inspired oxygen declines from 0.21 to 0.08. In addition, a linear relationship between brain PtO(2) and inspired O(2) levels was demonstrated r(2)=0.98 and r(2)=0.99 (pre- and post-acclimatization). Hypoxia acclimatization resulted in an increase in the overall brain PtO(2) by approximately 35%. This paper demonstrates the use of a novel chronically implanted fiber optic based sensor for measuring absolute PtO(2). It shows a very strong linear relationship in awake animals between inspired O(2) and tissue O(2), and shows that there is a proportional increase in PtO(2) over a range of inspired values after exposure to chronic hypoxia.
机译：组织氧合作用的水平提供了与生理条件下氧气输送，氧气利用，组织反应性和形态之间的平衡有关的信息。氧气的组织分压（PtO（2））受麻醉或约束作用的影响。这些因素可能会影响PtO（2）的绝对水平。在这项研究中，我们提出了一种新颖的光纤方法来测量大脑PtO（2）。此方法可用于未经麻醉，不受约束的动物，可提供PO（2）的绝对值，校准稳定，不消耗氧气，并且与MRI兼容。在急性缺氧期间以及高海拔适应28天之前和之后研究了大脑PtO（2）。将传感器长期植入八只Wistar大鼠的额皮质中。它由一个光纤探针组成，该探针的尖端含有与氧有关的荧光寿命。当吸入氧气的比例从0.21降至0.08时，大脑PtO（2）在适应前和适应后分别下降80％和76％。此外，大脑PtO（2）和受启发的O（2）水平之间的线性关系证明r（2）= 0.98和r（2）= 0.99（适应前和适应后）。缺氧适应使整个脑部PtO（2）增加约35％。本文演示了使用新型的基于长期植入的光纤的传感器来测量绝对PtO（2）。它显示了一个非常强的线性关系，在处于清醒状态的动物O（2）和组织O（2）之间，并且显示在暴露于慢性低氧后，PtO（2）在一系列激发值上成比例增加。

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