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首页> 外文期刊>Journal of Neuroscience Methods >3D-topography of cell nuclei in a vertebrate retina--a confocal and two-photon microscopic study.
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3D-topography of cell nuclei in a vertebrate retina--a confocal and two-photon microscopic study.

机译：脊椎动物视网膜中细胞核的3D地形图-共聚焦和双光子显微镜研究。

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摘要

We demonstrate methods to simultaneously acquire and evaluate the pattern of cell nuclei in the three cell layers of the vertebrate retina as an aspect of its functional morphology. 3D-position, shape and quantity of fluorescence-labelled cell nuclei are measured using laser scanning microscopy at several retinal locations, the pros and cons of single and two-photon excitation are compared. Subsequently topographies of all discriminable morphotypes are calculated via linear interpolation of local countings. In addition derived maps are calculated correlating density- and layer thickness-distributions to demonstrate the potential of 3D-morphometry in the retina. In the European anchovy Engraulis encrasicolus L. (Engraulididae, Teleostei) the angular density of all involved cell types varies considerably with the location in the hemispherical coordinate system. All cells belonging to the photopic system show a density peak in the ventro-temporal quadrant, suggesting acute vision in the frontal binocular visual field. A second, less pronounced maximum is found nasally.
机译：我们展示了同时获取和评估脊椎动物视网膜的三个细胞层中细胞核模式的方法，以此作为其功能形态学的一个方面。使用激光扫描显微镜在多个视网膜位置测量3D位置，荧光标记的细胞核的形状和数量，比较单光子和双光子激发的优缺点。随后，通过局部计数的线性插值计算所有可辨别形态型的形貌。此外，计算得出的图与密度和层厚度分布相关，以证明视网膜中3D形态的潜力。在欧洲an鱼Engraulis encrasicolus L.（Engraulididae，Teleostei）中，所有涉及的细胞类型的角密度都随半球坐标系中的位置而变化很大。属于明视系统的所有细胞均在腹-颞象限显示密度峰值，表明额叶双眼视野为急性视力。鼻部发现第二个不太明显的最大值。

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