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首页> 外文期刊>Journal of Neuroscience Methods >Stability of brain neocortical slice seizure-like activity during low-magnesium exposure: Measurement and effect of artificial cerebrospinal fluid temperature.
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Stability of brain neocortical slice seizure-like activity during low-magnesium exposure: Measurement and effect of artificial cerebrospinal fluid temperature.

机译：低镁暴露期间脑新皮质切片癫痫样活动的稳定性：人工脑脊液温度的测量和影响。

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摘要

Low-magnesium perfusion is an established method for inducing seizure-like activity in neocortical slices and is widely utilized as a model of human epilepsy. However, many contentious issues remain around optimizing in vitro cortical slice experimental protocols. In this study we investigated the stability of low-magnesium seizure-like event (SLE) activity at different perfusion temperatures; with an aim towards determining whether time-dependent effects in this preparation could be attributed to a reduction in tissue viability. The results showed that SLE characteristics changed significantly and consistently over a 3h recording period, independent of artificial cerebrospinal fluid temperature; there was a median increase in event frequency of 61% (p<0.001) following 2h of recording which remained elevated after 3h (52%, p<0.001). Event amplitude was significantly reduced after 3h (38% reduction, p<0.001). Similar effects were recorded whether perfusion temperature was maintained at 26 degrees C or 36 degrees C. No evidence was found to implicate a reduction in slice viability in these time-dependent changes in SLE behaviour. The results indicate that low-magnesium-induced SLE activity is not stable for long periods and the likelihood of time-related changes in slice performance must be considered when planning and interpreting experiments utilizing the low-magnesium seizure model.
机译：低镁灌注是一种在新皮层切片中诱导癫痫样活动的成熟方法，被广泛用作人类癫痫的模型。然而，围绕优化体外皮层切片实验方案仍存在许多争议性问题。在这项研究中，我们研究了在不同灌注温度下低镁癫痫样事件（SLE）活动的稳定性。目的在于确定该制剂中时间依赖性效应是否可归因于组织活力的降低。结果表明，在3h的记录期内，SLE的特征发生了显着且一致的变化，与人工脑脊液温度无关。在记录2小时后，事件发生频率的中位数增加了61％（p <0.001），而在3小时后仍保持了升高（52％，p <0.001）。 3h后事件振幅显着降低（降低38％，p <0.001）。无论灌注温度保持在26摄氏度还是36摄氏度，都记录了类似的效果。在这些与时间有关的SLE行为变化中，没有证据表明切片活力降低。结果表明，低镁诱发的SLE活性长期不稳定，在计划和解释使用低镁癫痫发作模型的实验时，必须考虑切片性能随时间变化的可能性。

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