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首页> 外文期刊>Journal of Neuroscience Methods >A novel method for inducing focal ischemia in vitro.
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A novel method for inducing focal ischemia in vitro.

机译：一种体外诱导局灶性缺血的新方法。

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Current in vitro models of stroke involve applying oxygen-glucose deprived (OGD) media over an entire brain slice or plate of cultured neurons. Thus, these models fail to mimic the focal nature of stroke as observed clinically and with in vivo rodent models of stroke. Our aim was to develop a novel in vitro brain slice model of stroke that would mimic focal ischemia and thus allow for the investigation of events occurring in the penumbra. This was accomplished by focally applying OGD medium to a small portion of a brain slice while bathing the remainder of the slice with normal oxygenated media. This technique produced a focal infarct on the brain slice that increased as a function of time. Electrophysiological recordings made within the flow of the OGD solution ("core") revealed that neurons rapidly depolarized (anoxic depolarization; AD) in a manner similar to that observed in other stroke models. Edaravone, a known neuroprotectant, significantly delayed this onset of AD. Electrophysiological recordings made outside the flow of the OGD solution ("penumbra") revealed that neurons within this region progressively depolarized throughout the 75 min of OGD application. Edaravone attenuated this depolarization and doubled neuronal survival. Finally, synaptic transmission in the penumbra was abolished within 50 min of focal OGD application. These results suggest that this in vitro model mimics events that occur during focal ischemia in vivo and can be used to determine the efficacy of therapeutics that target neuronal survival in the core and/or penumbra.
机译：当前的中风的体外模型涉及在整个神经切片或培养的神经元板上施加缺氧葡萄糖（OGD）培养基。因此，这些模型不能模仿临床和体内中风啮齿动物模型所观察到的中风的局灶性。我们的目的是开发一种新型的中风体外脑切片模型，该模型可以模拟局灶性局部缺血，从而可以对半影中发生的事件进行调查。这是通过将OGD介质集中施加到大脑切片的一小部分上，同时用正常的含氧介质冲洗其余部分来实现的。该技术在脑片上产生局灶性梗塞，并随时间增加。在OGD溶液（“核心”）流中进行的电生理记录表明，神经元以与其他中风模型中观察到的方式相似的方式快速去极化（缺氧性去极化; AD）。依达拉奉，一种已知的神经保护剂，显着延迟了AD的发作。在OGD溶液（“半影”）的流动之外进行的电生理记录显示，在整个OGD施加75分钟后，该区域内的神经元逐渐去极化。依达拉奉减弱了这种去极化作用，并使神经元存活率增加了一倍。最后，在局灶性OGD应用后50分钟内，半影中的突触传递被消除。这些结果表明，该体外模型模拟了体内局灶性局部缺血期间发生的事件，可用于确定靶向核心和/或半影神经元存活的疗法的功效。

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