• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Neuroscience Methods >Differentiation of mouse Neuro 2A cells into dopamine neurons.
【24h】

Differentiation of mouse Neuro 2A cells into dopamine neurons.

机译：小鼠Neuro 2A细胞分化为多巴胺神经元。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Neuro 2A (N2a) is a mouse neural crest-derived cell line that has been extensively used to study neuronal differentiation, axonal growth and signaling pathways. A convenient characteristic of these cells is their ability to differentiate into neurons within a few days. However, most differentiation methods reported for N2a cells do not provide information about the neuronal types obtained after each treatment. In this study, we evaluated the generation of N2a dopamine neurons following treatment with a number of factors known to induce neuronal differentiation. Our results showed that N2a cells express Nurr-related factor 1 (Nurr1) and produce low levels of tyrosine hydroxylase (TH) and dopamine. Both TH and dopamine levels were significantly enhanced in the presence of dibutyryl cyclic adenosine monophosphate (dbcAMP), as evidenced by Western blot, immunocytochemistry and high performance liquid chromatography (HPLC). In contrast to dbcAMP, other factors such as transforming growth factor beta1 (TGF beta 1), bone morphogenetic protein 4 (BMP4), glial cell-derived neurotrophic factor (GDNF) and retinoic acid (RA) did not increase TH expression. Further investigation confirmed that the effect of dbcAMP on production of TH-positive neurons was mediated through cyclic AMP (cAMP) responsive element binding protein (CREB) and it was antagonized by RA. Thus, although various treatments can be used to generate N2a neurons, only dbcAMP significantly enhanced the formation of dopamine neurons. Taken together, this study provided a simple and reliable method to generate dopamine neurons for rapid and efficient physiological and pharmacological assays.
机译：Neuro 2A（N2a）是一种源自小鼠神经rest的细胞系，已广泛用于研究神经元分化，轴突生长和信号传导途径。这些细胞的便利特征是它们在几天内分化为神经元的能力。但是，大多数报道的N2a细胞分化方法均未提供每次治疗后获得的神经元类型的信息。在这项研究中，我们评估了用许多已知诱导神经元分化的因子治疗后N2a多巴胺神经元的生成。我们的结果表明N2a细胞表达Nurr相关因子1（Nurr1），并产生低水平的酪氨酸羟化酶（TH）和多巴胺。 Western印迹法，免疫细胞化学法和高效液相色谱法（HPLC）证明，在存在二丁酰基环一磷酸腺苷（dbcAMP）的情况下，TH和多巴胺水平均显着提高。与dbcAMP相比，其他因子，例如转化生长因子beta1（TGF beta 1），骨形态发生蛋白4（BMP4），神经胶质细胞源性神经营养因子（GDNF）和视黄酸（RA）不会增加TH表达。进一步的研究证实，dbcAMP对TH阳性神经元产生的影响是通过环状AMP（cAMP）响应元件结合蛋白（CREB）介导的，并被RA拮抗。因此，尽管可以使用各种方法来生成N2a神经元，但只有dbcAMP可以显着增强多巴胺神经元的形成。两者合计，这项研究提供了一种简单而可靠的方法来生成多巴胺神经元，以进行快速有效的生理和药理分析。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号