• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Neuroscience Methods >Paraffin-embedded tissue (PET) blot method: application to Alzheimer disease.
【24h】

Paraffin-embedded tissue (PET) blot method: application to Alzheimer disease.

机译：石蜡包埋组织（PET）印迹方法：适用于阿尔茨海默氏病。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Traditionally, immunohistochemistry, immunoblotting, and histoblot have been used to detect protein in tissue samples. However, each of these techniques has a number of disadvantages. The sensitivity of protein detection in immunohistochemistry is lost due to fixation or paraffin embedding methods that modify antigenic sites. The anatomical resolution and specific cellular involvement are lost in immunoblotting. Histoblot, a hybrid of these two techniques, is able to resolve these issues, but it cannot be applied to formalin-fixed tissues. A recent technique, paraffin-embedded tissue (PET) blot, retains the superior protein detection and anatomical resolution of histoblot and is applicable to formalin-fixed tissues. Unfortunately, a major obstacle to the widespread application of PET is the lack of a detailed methodological description. In this paper, we describe a PET blotting method that was formulated from our own empirical and experimental research in Alzheimer disease and a systematic review of the current literature. From this, we conclude that PET can be applied to a variety of conditions with a wide spectrum of pathology.
机译：传统上，免疫组织化学，免疫印迹和组织印迹已用于检测组织样品中的蛋白质。但是，这些技术中的每一个都有许多缺点。免疫组织化学中蛋白质检测的敏感性由于修饰或修饰抗原位点的石蜡包埋方法而丧失。在免疫印迹中丢失了解剖学分辨率和特定的细胞参与。这两种技术的组合，Histoblot能够解决这些问题，但不能应用于福尔马林固定的组织。石蜡包埋的组织（PET）印迹是一种最新技术，保留了组织印迹的卓越蛋白质检测和解剖分辨率，适用于福尔马林固定的组织。不幸的是，PET广泛应用的主要障碍是缺乏详细的方法学描述。在本文中，我们描述了一种PET印迹方法，该方法是根据我们自己在阿尔茨海默氏病的经验和实验研究以及当前文献的系统综述而制定的。由此，我们得出结论，PET可以应用于多种病理类型的疾病。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号